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Invitrogen
Phospho-PKM2 (Tyr370) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-PKM2 (Tyr370) Antibody (PA5-105498) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-PKM2 (Tyr370) in human gastric cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-PKM2 (Tyr370) polyclonal antibody (Product # PA5-105498) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105498
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PKM(Accession P14618), corresponding to amino acid residues around phosphorylated Tyr370.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of PKM2 only when phosphorylated at Tyr370.
Target Information
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CTHBP; Cytosolic thyroid hormone-binding protein; cytosolic thyroid hormone-binding protein (EC 2.7.1.40); epididymis secretory protein Li 30; isozymes M1/M2; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; similar to human pyruvate kinase M2; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; Tumor M2-PK; Tyrosine-protein kinase PKM2; unnamed protein product
Gene Aliases: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; p58; Pk-2; Pk-3; PK2; PK3; PKM; PKM12; PKM2; Pykm; TCB; THBP1
UniProt ID: (Human) P14618, (Mouse) P52480, (Rat) P11980
Entrez Gene ID: (Human) 5315, (Mouse) 18746, (Rat) 25630
nucleotide binding
magnesium ion binding
transcription coactivator activity
RNA binding
mRNA binding
catalytic activity
protein tyrosine kinase activity
pyruvate kinase activity
protein binding
ATP binding
kinase activity
transferase activity
MHC class II protein complex binding
potassium ion binding
histone kinase activity (H3-T11 specific)
protein homodimerization activity
cadherin binding
metal ion binding
ADP binding
poly(A) RNA binding
kinase
glycolytic process
chromatin remodeling
regulation of translation
programmed cell death
cellular response to insulin stimulus
positive regulation of transcription from RNA polymerase II promoter
canonical glycolysis
positive regulation of sprouting angiogenesis
positive regulation of cytoplasmic translation
liver development
glucose metabolic process
ATP biosynthetic process
metabolic process
phosphorylation
organ regeneration
pyruvate biosynthetic process
skeletal muscle tissue regeneration
response to hypoxia
response to nutrient
response to gravity
response to organic substance
response to muscle inactivity
response to insulin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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