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Invitrogen
Phospho-PLK1 (Tyr217) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-PLK1 (Tyr217) Antibody (PA5-105590) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-PLK1 (Tyr217) in rat lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-PLK1 (Tyr217) polyclonal antibody (Product # PA5-105590) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105590
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PLK1(Accession P53350), corresponding to amino acid residues around phosphorylated Tyr217.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of PLK1 only when phosphorylated at Tyr217.
Target Information
PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PLK 1; PLK-1; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14
Gene Aliases: PLK; PLK1; STPK13
UniProt ID: (Mouse) Q07832, (Rat) Q62673
Entrez Gene ID: (Mouse) 18817, (Rat) 25515
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
microtubule binding
anaphase-promoting complex binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
non-receptor serine/threonine protein kinase
protein modifying enzyme
mitotic sister chromatid segregation
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
mitotic cytokinesis
cytokinesis
microtubule bundle formation
protein phosphorylation
cell cycle
mitotic nuclear division
mitotic spindle assembly checkpoint
regulation of mitotic cell cycle
positive regulation of peptidyl-threonine phosphorylation
phosphorylation
female meiosis chromosome segregation
protein ubiquitination
peptidyl-serine phosphorylation
regulation of mitotic metaphase/anaphase transition
G2 DNA damage checkpoint
protein destabilization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
polar body extrusion after meiotic divisions
negative regulation of apoptotic process
regulation of protein binding
homologous chromosome segregation
establishment of protein localization
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of proteolysis
centrosome organization
cell division
positive regulation of ubiquitin-protein transferase activity
synaptonemal complex disassembly
protein localization to chromatin
regulation of mitotic spindle assembly
positive regulation of ubiquitin protein ligase activity
response to antibiotic
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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