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Invitrogen
Phospho-VPS34 (Ser244, Ser249) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-VPS34 (Ser244, Ser249) Antibody (PA5-105679) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-VPS34 (Ser244, Ser249) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-VPS34 (Ser244, Ser249) polyclonal antibody (Product # PA5-105679) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105679
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PIK3C3(Accession Q8NEB9), corresponding to amino acid residues around phosphorylated Ser249.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of PI3 Kinase Class III only when phosphorylated at Ser244/249.
Target Information
PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: catalytic phosphatidylinositol 3-kinase 3; catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; phosphatidylinositol 3-kinase p100 subunit; phosphoinositide-3-kinase, class 3; PI-3K Vps34p; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3; unnamed protein product; vacuolar protein sorting 34 homolog
Gene Aliases: 5330434F23Rik; hVps34; VPS34
UniProt ID: (Human) Q8NEB9, (Mouse) Q6PF93, (Rat) O88763
Entrez Gene ID: (Human) 5289, (Mouse) 225326, (Rat) 65052
autophagosome assembly
pexophagy
protein targeting to lysosome
endocytosis
autophagy
endosome organization
regulation of autophagy
macroautophagy
regulation of macroautophagy
protein processing
regulation of cytokinesis
phosphatidylinositol-3-phosphate biosynthetic process
cellular response to glucose starvation
response to leucine
protein kinase B signaling
positive regulation by host of viral genome replication
early endosome to late endosome transport
positive regulation of natural killer cell mediated cytotoxicity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
synaptic vesicle endocytosis
autophagosome maturation
positive regulation of protein lipidation
protein phosphorylation
cellular response to starvation
protein localization to pre-autophagosomal structure
interferon-gamma-mediated signaling pathway
interleukin-6-mediated signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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