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Invitrogen

Phospho-TWIST1 (Ser68) Polyclonal Antibody

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View all (34) TWIST1 antibodies

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Phospho-TWIST1 (Ser68) Polyclonal Antibody

Product Details

PA5-105703

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human TWIST1(Accession Q15672), corresponding to amino acid residues around phosphorylated Ser68.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2817131

Product Specific Information

Antibody detects endogenous levels of Twist1 only when phosphorylated at Ser68.

Target Information

TWIST1 acts as a transcriptional regulator. It inhibits myogensis by sequestering E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B. TWIST also regulates cranial suture patterning and fusion and gene expression differentially depending on dimer composition. Mutations in the gene are implicated in Saethre-Chotzen syndrome, a disease characterized by coronal synostosis, brachycephaly, low front hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: B-HLH DNA binding protein; class A basic helix-loop-helix protein 38; H-twist; p ACS3; twist basic helix-loop-helix transcription factor 1; twist homolog 1; TWIST homolog of drosophila

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Gene Aliases: ACS3; bHLHa38; BPES2; BPES3; CRS; CRS1; CSO; SCS; SWCOS; TWIST

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UniProt ID: (Human) Q15672

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Entrez Gene ID: (Human) 7291

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription cofactor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding protein domain specific binding identical protein binding protein homodimerization activity histone deacetylase binding bHLH transcription factor binding protein dimerization activity E-box binding DNA-binding transcription factor binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ossification aortic valve morphogenesis mitral valve morphogenesis regulation of transcription from RNA polymerase II promoter positive regulation of gene expression positive regulation of epithelial to mesenchymal transition positive regulation of cell migration regulation of bone mineralization positive regulation of fatty acid beta-oxidation developmental process positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production embryonic hindlimb morphogenesis negative regulation of peroxisome proliferator activated receptor signaling pathway outer ear morphogenesis embryonic digit morphogenesis negative regulation of DNA damage response, signal transduction by p53 class mediator negative regulation of cell differentiation negative regulation of osteoblast differentiation positive regulation of angiogenesis negative regulation of striated muscle tissue development negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic cranial skeleton morphogenesis negative regulation of protein kinase B signaling cranial suture morphogenesis embryonic camera-type eye formation eyelid development in camera-type eye cellular response to hypoxia positive regulation of monocyte chemotactic protein-1 production energy homeostasis negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of DNA-templated transcription, initiation positive regulation of cell motility negative regulation of cellular senescence negative regulation of double-strand break repair cell proliferation involved in heart valve development positive regulation of endocardial cushion to mesenchymal transition involved in heart valve formation
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