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Invitrogen
Phospho-CD31 (Tyr690) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-CD31 (Tyr690) Antibody (PA5-105759) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-CD31 (Tyr690) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-CD31 (Tyr690) polyclonal antibody (Product # PA5-105759) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105759
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PECAM1(Accession P16284), corresponding to amino acid residues around phosphorylated Tyr690.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of CD31 only when phosphorylated at Tyr690.
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adhesion molecule; CD 31; CD31 antigen; PECAM-1; PECAM-1 precursor; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule 1; unnamed protein product
Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; Pecam; PECAM-1
UniProt ID: (Human) P16284, (Rat) Q3SWT0, (Mouse) Q08481
Entrez Gene ID: (Human) 5175, (Rat) 29583, (Mouse) 18613
angiogenesis
endothelial cell morphogenesis
positive regulation of leukocyte migration
positive regulation of cellular extravasation
phagocytosis
immune response
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
leukocyte cell-cell adhesion
cell surface receptor signaling pathway
Rho protein signal transduction
regulation of cell migration
positive regulation of cell migration
negative regulation of actin filament polymerization
negative regulation of GTPase activity
monocyte extravasation
wound healing
T cell activation
vasodilation
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of MAPK cascade
endothelial cell migration
negative regulation of peptidyl-tyrosine phosphorylation
diapedesis
detection of mechanical stimulus
positive regulation of protein kinase B signaling
establishment of endothelial barrier
bicellular tight junction assembly
cellular response to mechanical stimulus
cellular response to interferon-gamma
cellular response to transforming growth factor beta stimulus
glomerular endothelium development
neutrophil extravasation
endothelial cell-matrix adhesion
cell-cell adhesion
positive regulation of protein localization to cell-cell junction
positive regulation of intracellular signal transduction
blood vessel development
endothelial cell development
signal transduction
cell migration
cell recognition
maintenance of blood-brain barrier
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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