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Invitrogen
Phospho-Ataxin 3 (Ser256) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-Ataxin 3 (Ser256) Antibody (PA5-105967) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-Ataxin 3 (Ser256) in human lung cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Ataxin 3 (Ser256) polyclonal antibody (Product # PA5-105967) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105967
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATXN3(Accession P54252), corresponding to amino acid residues around phosphorylated Ser256.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Ataxin 3 only when phosphorylated at Ser256.
Target Information
Machado-Joseph disease is an autosomal dominant neurologic disorder, and is now known to be the same as previously described spinocerebellar ataxia-3. MJD protein (Ataxin-3) contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. This protein interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription, and also acts as a histone-binding protein that regulates transcription. MJD is a deubiquitinating enzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ataxin3; josephin; Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) homolog; Machado-Joseph disease protein 1; machado-Joseph disease protein 1 homolog; olivopontocerebellar ataxia 3; spin; spinocerebellar ataxia type 3 protein; unnamed protein product
Gene Aliases: 2210008M02Rik; AT3; ataxin-3; ATX3; JOS; MJD; MJD1; Rsca3; SCA3
UniProt ID: (Human) P54252, (Rat) O35815, (Mouse) Q9CVD2
Entrez Gene ID: (Human) 4287, (Rat) 60331, (Mouse) 110616
thiol-dependent ubiquitin-specific protease activity
protein binding
ubiquitin protein ligase binding
identical protein binding
ATPase binding
Lys63-specific deubiquitinase activity
Lys48-specific deubiquitinase activity
RNA polymerase II regulatory region sequence-specific DNA binding
transcription corepressor binding
histone deacetylase activity
histone deacetylase binding
microtubule cytoskeleton organization
nucleotide-excision repair
ubiquitin-dependent protein catabolic process
misfolded or incompletely synthesized protein catabolic process
chemical synaptic transmission
nervous system development
regulation of cell-substrate adhesion
protein deubiquitination
actin cytoskeleton organization
cellular response to amino acid starvation
cellular response to heat
monoubiquitinated protein deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
intermediate filament cytoskeleton organization
protein K63-linked deubiquitination
protein K48-linked deubiquitination
cellular response to misfolded protein
negative regulation of TORC1 signaling
positive regulation of ERAD pathway
protein localization to cytosolic proteasome complex
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
chromatin remodeling
exploration behavior
cytoplasmic translation
cellular response to starvation
cellular response to nutrient levels
TORC1 signaling
negative regulation of translational initiation
positive regulation of translational initiation
positive regulation of TORC1 signaling
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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