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Invitrogen
Phospho-E2F1 (Ser364) Polyclonal Antibody
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Phospho-E2F1 (Ser364) Antibody (PA5-106084) in IHC (P)
Immunohistochemistry analysis of Phospho-E2F1 (Ser364) in human kidney cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-E2F1 (Ser364) polyclonal antibody (Product # PA5-106084) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-106084
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human E2F1(Accession Q01094), corresponding to amino acid residues around phosphorylated Ser364.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of E2F1 only when phosphorylated at Ser364.
靶标信息
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E2F-1; PBR3; pRB-binding protein E2F-1; RBAP-1; RBBP-3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1
基因别名: E2F-1; E2F1; mKIAA4009; RBBP3
UniProt ID: (Mouse) Q61501, (Rat) O09139
Entrez Gene ID: (Mouse) 13555, (Rat) 399489
core promoter binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
protein kinase binding
sequence-specific DNA binding
transcription regulatory region DNA binding
RNA metabolism protein
nucleic acid metabolism protein
general transcription factor
DNA damage checkpoint
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
apoptotic process
cell cycle
intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of gene expression
forebrain development
anoikis
negative regulation of DNA binding
negative regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
mRNA stabilization
regulation of cell cycle
negative regulation of fat cell proliferation
cellular response to xenobiotic stimulus
negative regulation of transcription involved in G1/S transition of mitotic cell cycle
intrinsic apoptotic signaling pathway by p53 class mediator
lens fiber cell apoptotic process
cellular response to nerve growth factor stimulus
regulation of G1/S transition of mitotic cell cycle
G1/S transition of mitotic cell cycle
spermatogenesis
cellular response to fatty acid
cellular response to hypoxia
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