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Invitrogen
SnoN Polyclonal Antibody
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图: 1 / 3
SnoN Antibody (PA5-106469) in ICC/IF
Immunofluorescent analysis of SnoN in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SnoN polyclonal antibody (Product # PA5-106469) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-106469
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SKIL(Accession P12757), corresponding to amino acid residues Q632-A682.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total SKIL.
靶标信息
TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: SKI-like oncogene; Ski-like protein; Ski-related oncogene; Ski-related protein; ski/sno related
基因别名: SKIL; Skir; SNO; SnoN
UniProt ID: (Mouse) Q60665
Entrez Gene ID: (Rat) 114208, (Mouse) 20482
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
molecular_function
DNA binding
chromatin binding
transcription corepressor activity
protein domain specific binding
protein complex binding
SMAD binding
protein binding
negative regulation of transcription from RNA polymerase II promoter
blastocyst formation
lymphocyte homeostasis
cell cycle arrest
transforming growth factor beta receptor signaling pathway
spermatogenesis
skeletal muscle tissue development
negative regulation of transforming growth factor beta receptor signaling pathway
response to cytokine
regulation of apoptotic process
negative regulation of cell differentiation
response to antibiotic
positive regulation of axonogenesis
protein homotrimerization
protein heterotrimerization
lens fiber cell differentiation
response to growth factor
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage
transcription from RNA polymerase II promoter
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