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Invitrogen
EGLN1/EGLN2 Polyclonal Antibody
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FIGURE: 1 / 1
EGLN1/EGLN2 Antibody (PA5-109677) in WB
Western blot analysis of EGLN1/EGLN2 in extracts of various cell lines. Samples were incubated with EGLN1/EGLN2 Polyclonal antibody (Product # PA5-109677) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-109677
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.81 mg/mL
Amount
81 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: DIRGDKITWI EGKEPGCETI GLLMSSMDDL IRHCNGKLGS YKINGRTKAM VACYPGNGTG YVRHVDNPNG DGRCVTCIYY LNKDWDAKVS GGILRIFPEG K
Target Information
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C1orf12; chromosome 1 open reading frame 12; egl nine homolog 2; egl nine-like protein 1; estrogen-induced tag 6; HIF-prolyl hydroxylase 1; HIF-prolyl hydroxylase 2; hypoxia-inducible factor prolyl hydroxylase 1; hypoxia-inducible factor prolyl hydroxylase 2; NB4 apoptosis/differentiation related protein; nuclear protein EIT-6; prolyl hydroxylase domain-containing protein 1; prolyl hydroxylase domain-containing protein 2; unnamed protein product; zinc finger MYND domain-containing protein 6
Gene Aliases: C1orf12; ECYT3; EIT-6; EIT6; HALAH; HIF-PH1; HIF-PH2; HIFPH1; HIFPH2; HPH-1; HPH-2; HPH-3; HPH2; PHD1; PHD2; SM20; ZMYND6
UniProt ID: (Human) Q9GZT9, (Human) Q96KS0
Entrez Gene ID: (Human) 54583, (Human) 112398
enzyme inhibitor activity
iron ion binding
protein binding
ferrous iron binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
enzyme binding
L-ascorbic acid binding
peptidyl-proline dioxygenase activity
peptidyl-proline 4-dioxygenase activity
hypoxia-inducible factor-proline dioxygenase activity
oxygen sensor activity
regulation of cell growth
response to hypoxia
peptidyl-proline hydroxylation to 4-hydroxy-L-proline
intracellular estrogen receptor signaling pathway
regulation of neuron apoptotic process
cell redox homeostasis
positive regulation of protein catabolic process
cellular response to hypoxia
cellular iron ion homeostasis
oxygen homeostasis
regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
response to nitric oxide
regulation of modification of postsynaptic structure
regulation protein catabolic process at postsynapse
negative regulation of hypoxia-inducible factor-1alpha signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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