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H4K12su Polyclonal Antibody
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FIGURE: 1 / 2
H4K12su Antibody (PA5-112553) in ICC/IF
Immunocytochemichal analysis of Succinyl-Histone H4 (Lys12) in Hela cells using a Polyclonal antibody (Product # PA5-112553) at a dilution of 1:5. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-112553
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide sequence around site of Succinyl-Lys (12) derived from Human Histone H4
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.14 mg/mL
Amount
14 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
H4K12su is the SUMOylation of lysine 12 on histone H4, a core nucleosomal protein (UniProt histone H4 family) involved in chromatin structure. This modification is associated with transcriptional repression by inhibiting activating marks like acetylation and H3K4 methylation. Despite promoting a more open chromatin structure, it functions as a regulatory epigenetic mark that fine-tunes gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H4; H4 histone family, member A; H4 histone family, member B; H4 histone family, member C; H4 histone family, member D; H4 histone family, member E; H4 histone family, member G; H4 histone family, member H; H4 histone family, member I; H4 histone family, member J; H4 histone family, member K; H4 histone family, member L; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K12; H4 K12su; H4K12; H4Lys12su; HIST1H4F; HIST1H4H; HIST2H4; histone 1, H4a; histone 1, H4b; histone 1, H4c; histone 1, H4d; histone 1, H4e; histone 1, H4f; histone 1, H4g; histone 1, H4h; histone 1, H4i; histone 1, H4j; histone 1, H4k; histone 1, H4l; histone 2, H4a; histone 2, H4b; Histone 3; Histone 4 family, member M; histone 4, H4; histone cluster 1 H4 family member a; histone cluster 1 H4 family member b; histone cluster 1 H4 family member c; histone cluster 1 H4 family member d; histone cluster 1 H4 family member e; histone cluster 1 H4 family member f; histone cluster 1 H4 family member g; histone cluster 1 H4 family member h; histone cluster 1 H4 family member i; histone cluster 1 H4 family member j; histone cluster 1 H4 family member k; histone cluster 1 H4 family member l; histone cluster 1, H4a; histone cluster 1, H4b; histone cluster 1, H4c; histone cluster 1, H4d; histone cluster 1, H4e; histone cluster 1, H4f; histone cluster 1, H4g; histone cluster 1, H4h; histone cluster 1, H4i; histone cluster 1, H4j; histone cluster 1, H4k; histone cluster 1, H4l; histone cluster 2 H4 family member a; histone cluster 2 H4 family member b; histone cluster 2, H4a; histone cluster 2, H4b; histone cluster 4 H4; histone family member; Histone H3; Histone H4K12; Histone H4K12su; histone IV, family 2; unnamed protein product
Gene Aliases: dJ160A22.1; dJ160A22.2; dJ221C16.1; dJ221C16.9; FO108; H4; H4-16; H4.k; H4/b; H4/c; H4/d; H4/e; H4/g; H4/h; H4/I; H4/j; H4/k; H4/l; H4/m; H4/n; H4/o; H4/p; H4F2; H4F2iii; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FL; H4FM; H4FN; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4G; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; TEBIVANED1; TEBIVANED2; TEBIVANED3; TEBIVANED4; TEVANED1; TEVANED2; TEVANED3; TEVANED4
UniProt ID: (Human) P62805, (Human) Q99525
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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