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Invitrogen
MR1 Polyclonal Antibody
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FIGURE: 1 / 3
MR1 Antibody (PA5-114362) in WB
Western blot analysis of MR1 in the following samples: Lane 1: human T-47D whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human A431 whole cell lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash) and blocked with 5% Non-fat Milk/TBS (1.5 hrs., room temperature). The membrane was incubated in MR1 pol... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114362
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MR1 recombinant protein (Position: R23-D269).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human T-47D whole cell, human U-937 whole cell, human A431 whole cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T- cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Class I histocompatibility antigen-like protein; histocompatibility-2 complex class 1-like sequence; major histocompatibility complex class I-related gene protein; Major histocompatibility complex class I-related protein 1; MCH class I; MHC class I-like sequence; MHC class I-related gene protein; MHC class I-related protein 1; MHC class-I related-gene protein
Gene Aliases: H2ls; Hlals; MR1; Mr1a
UniProt ID: (Mouse) Q8HWB0, (Rat) O19477
Entrez Gene ID: (Mouse) 15064, (Rat) 25119
cytokine production involved in immune response
immune system process
antigen processing and presentation of peptide antigen via MHC class I
immune response
antigen processing and presentation
interleukin-1 beta production
interleukin-17 production
innate immune response
defense response to Gram-negative bacterium
positive regulation of T cell mediated cytotoxicity
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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