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Invitrogen
DHCR7 Polyclonal Antibody
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FIGURE: 1 / 2
DHCR7 Antibody (PA5-115116) in ICC/IF
Immunocytochemistry analysis of DHCR7 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115116) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115116
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DHCR7(Accession Q9UBM7), corresponding to amino acid residues M1-P51.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total DHCR7.
Target Information
Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 7-DHC reductase; cholesterol-5,6-epoxide hydrolase subunit DHCR7; D7SR; delta-7-dehydrocholesterol reductase; delta7-sterol reductase; Dhcr7; putative sterol reductase SR-2; sterol Delta(7)-reductase; sterol delta-7-reductase; sterol reductase; sterol reductase SR-2; unnamed protein product
Gene Aliases: SLOS
UniProt ID: (Human) Q9UBM7, (Rat) Q9Z2Z8, (Mouse) O88455
Entrez Gene ID: (Human) 1717, (Rat) 64191, (Mouse) 13360
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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