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Invitrogen
ERK2 Polyclonal Antibody
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FIGURE: 1 / 2
ERK2 Antibody (PA5-115194) in IHC (P)
Immunohistochemistry analysis of ERK2 in human kidney tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115194) at a dilution of 1:100. Secondary staining was applied with HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115194
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAPK1(Accession P28482), corresponding to amino acid residues D179-P229.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ERK2.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ERK-2; ERT1; Extracellular signal-regulated kinase 2; extracellular-signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; mitogen activated protein kinase 1; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p42-MAPK
Gene Aliases: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK2; Mapk; MAPK1; MAPK2; p41mapk; p42mapk; PRKM1; PRKM2
UniProt ID: (Mouse) P63085, (Rat) P63086
Entrez Gene ID: (Mouse) 26413, (Rat) 116590
nucleotide binding
phosphotyrosine binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
transcription factor binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
phosphatase binding
mitogen-activated protein kinase kinase kinase binding
non-receptor serine/threonine protein kinase
MAPK cascade
MAPK import into nucleus
transcription, DNA-templated
protein phosphorylation
apoptotic process
cellular response to DNA damage stimulus
cell cycle
signal transduction
response to toxic substance
organ morphogenesis
positive regulation of peptidyl-threonine phosphorylation
phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
sensory perception of pain
cytosine metabolic process
lipopolysaccharide-mediated signaling pathway
response to lipopolysaccharide
regulation of stress-activated MAPK cascade
mammary gland epithelial cell proliferation
intracellular signal transduction
ERBB signaling pathway
response to exogenous dsRNA
response to estrogen
negative regulation of cell differentiation
positive regulation of translation
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of sequence-specific DNA binding transcription factor activity
regulation of cytoskeleton organization
long-term synaptic potentiation
lung morphogenesis
trachea formation
labyrinthine layer blood vessel development
ERK1 and ERK2 cascade
response to epidermal growth factor
cellular response to organic substance
caveolin-mediated endocytosis
regulation of Golgi inheritance
cellular response to granulocyte macrophage colony-stimulating factor stimulus
regulation of early endosome to late endosome transport
positive regulation of cell proliferation
positive regulation of cell migration
regulation of protein stability
positive regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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