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Invitrogen
Laminin Polyclonal Antibody
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FIGURE: 1 / 7
Laminin Antibody (PA5-115490) in ICC/IF
Immunocytochemistry analysis of Laminin in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115490) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115490
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LAMA2(Accession P24043), corresponding to amino acid residues Q383-A433.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Laminin 2 alpha.
Target Information
Laminin is a crucial glycoprotein that plays a significant role in the extracellular matrix, particularly within the basement membrane. It is composed of three subunits: alpha, beta, and gamma chains. These chains are disulfide-linked polypeptides that together form a heterotrimer, resembling the shape of a cross. The human genome encodes 11 genetically distinct laminin chains, which combine to form over 14 different laminin isoforms, each with specific tissue distributions and functions. Laminins can bind to other laminin molecules, as well as integrins and other matrix proteins, providing structural support and influencing cell differentiation, migration, and adhesion. These glycoproteins are vital during early embryonic development, where they contribute to the formation of basement membrane-like structures, even in the absence of type IV collagen. The evolutionary origin of laminin gene families can be traced back to early branching animals, providing insights into metazoan gene family development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dystrophia muscularis; laminin A chain; laminin A-chain; laminin B1; laminin B1 chain; laminin B1 subunit 1; laminin B2 chain; laminin B2 precursor; laminin M chain; laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, gamma 1; laminin, gamma 1 (formerly LAMB2); laminin-1; laminin-1 subunit alpha; laminin-1 subunit beta; laminin-1 subunit gamma; laminin-10 subunit beta; laminin-10 subunit gamma; laminin-11 subunit gamma; laminin-111; laminin-12 subunit alpha; laminin-12 subunit beta; laminin-2 subunit alpha; laminin-2 subunit beta; laminin-2 subunit gamma; laminin-3 subunit alpha; laminin-3 subunit gamma; laminin-4 subunit alpha; laminin-4 subunit gamma; laminin-6 subunit beta; laminin-6 subunit gamma; laminin-7 subunit gamma; laminin-8 subunit beta; laminin-8 subunit gamma; laminin-9 subunit gamma; merosin heavy chain; mutant laminin subunit alpha 2; S-LAM alpha; S-LAM gamma; S-laminin subunit alpha; S-laminin subunit gamma; unnamed protein product
Gene Aliases: 5830440B04; B2e; CLM; D130003D08Rik; dy; LAMA; Lamb-1; Lamb1-1; LAMB2; LAMM; LIS5; LKBMH; LUCAO; MDC1A; mer; merosin; mKIAA4087; PTBHS; S-LAM-alpha
UniProt ID: (Human) P25391, (Human) P24043, (Human) P07942, (Human) P11047, (Mouse) P19137, (Mouse) Q60675, (Mouse) P02469
Entrez Gene ID: (Human) 284217, (Human) 3908, (Human) 3912, (Human) 3915, (Rat) 316758, (Mouse) 16772, (Rat) 309368, (Mouse) 16773, (Mouse) 16777, (Rat) 298941, (Rat) 117036, (Mouse) 226519
cell adhesion
axon guidance
organ morphogenesis
tissue development
Schwann cell differentiation
regulation of cell adhesion
regulation of cell migration
regulation of synaptic transmission, cholinergic
positive regulation of synaptic transmission, cholinergic
regulation of embryonic development
signal transduction
cell surface receptor signaling pathway
nervous system development
negative regulation of muscle cell apoptotic process
positive regulation of Rac protein signal transduction
positive regulation of cell adhesion
positive regulation of muscle cell differentiation
positive regulation of protein kinase B signaling
regulation of basement membrane organization
positive regulation of skeletal muscle acetylcholine-gated channel clustering
positive regulation of integrin-mediated signaling pathway
negative regulation of cell adhesion
neuronal-glial interaction involved in cerebral cortex radial glia guided migration
positive regulation of cell migration
neuron projection development
substrate adhesion-dependent cell spreading
endodermal cell differentiation
odontogenesis
positive regulation of epithelial cell proliferation
chromatin organization
gene expression
cell migration
extracellular matrix disassembly
hair follicle morphogenesis
hemidesmosome assembly
hair cell differentiation
tissue morphogenesis
protein-containing complex assembly
hair follicle cell proliferation
morphogenesis of a polarized epithelium
morphogenesis of an epithelial sheet
brain development
biological_process
lung development
camera-type eye development
establishment of epithelial cell apical/basal polarity
blood vessel morphogenesis
branching morphogenesis of an epithelial tube
retina development in camera-type eye
epithelial tube branching involved in lung morphogenesis
branching involved in salivary gland morphogenesis
retinal blood vessel morphogenesis
circulatory system development
embryo implantation
learning or memory
basement membrane assembly
muscle organ development
maintenance of blood-brain barrier
endoderm development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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