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Invitrogen
Phospho-XRCC1 (Thr284) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-XRCC1 (Thr284) Antibody (PA5-118790) in ICC/IF
Immunocytochemistry analysis of Phospho-XRCC1 (Thr284) in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-XRCC1 (Thr284) polyclonal antibody (Product # PA5-118790) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an Alex... View More
Product Details
PA5-118790
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human XRCC1(Accession P18887), corresponding to amino acid residues around phosphorylated Thr284.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of XRCC1 only when phosphorylated at Thr284.
Target Information
XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA repair protein XRCC1; DNA-repair protein; unnamed protein product; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross-complementing protein 1; XRCC1
Gene Aliases: RCC; SCAR26; XRCC1
UniProt ID: (Human) P18887
Entrez Gene ID: (Human) 7515
single strand break repair
DNA repair
base-excision repair
double-strand break repair
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
negative regulation of protein ADP-ribosylation
hippocampus development
response to hydroperoxide
telomeric DNA-containing double minutes formation
regulation of base-excision repair
negative regulation of protection from non-homologous end joining at telomere
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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