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Invitrogen
ATG14 Polyclonal Antibody
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FIGURE: 1 / 4
ATG14 Antibody (PA5-119797) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse testis tissue using ATG14 Polyclonal Antibody (Product # PA5-119797). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the ATG14 antibody at a dilution of ... View More
Product Details
PA5-119797
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human ATG14L aa 421-456.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Hela cell lysate, MCF-7 cell lysate, HepG2 cell lysate, SiHa cell lysate, rat brain tissue, human colon tissue, mouse testis tissue.
Subcellular Location: Endoplasmic reticulum membrane, Cytoplasm, Preautophagosomal structure membrane, autophagosome membrane.
Target Information
ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene and is located on chromosome 14 in humans. It is also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator). Autophagy is an evolutionarily conserved process that involves recycling of misfolded cellular proteins and degradation of dysfunctional organelles. Autophagy is induced as a cellular response to nutrient stress which includes the formation of autophagosomes, fusion of these with the lysosome and formation of the autophagolysosome. The Class III PI3-Kinase, Vps34 and its interacting partner Beclin 1 have been shown to form a complex which specifically includes ATG 14 (type 1) or UVRAG (type 2). Atg 14 guides the type 1 complex to the preautophagosomal structure (PAS) and is critical for the initiation of autophagosome formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATG14 autophagy related 14 homolog; Atg14-like protein; Atg14L; Autophagy-related protein 14-like protein; Bakor; Barkor; Beclin 1-associated autophagy-related key regulator; Beclin 1-Interacting protein; unnamed protein product; VATG14 autophagy related 14 homolog
Gene Aliases: 4832427M01; ATG14; ATG14L; BARKOR; D14Ertd114e; D14Ertd436e; KIAA0831; RGD1304610
UniProt ID: (Human) Q6ZNE5, (Mouse) Q8CDJ3, (Rat) D4A4K3
Entrez Gene ID: (Human) 22863, (Mouse) 100504663, (Rat) 305831
autophagosome assembly
mitophagy
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
protein targeting to lysosome
autophagy
endosome to lysosome transport
cellular response to starvation
posttranscriptional regulation of gene expression
macroautophagy
autophagosome membrane docking
regulation of macroautophagy
phosphatidylinositol-3-phosphate biosynthetic process
cellular response to glucose starvation
protein kinase B signaling
early endosome to late endosome transport
innate immune response
defense response to virus
regulation of protein complex stability
regulation of triglyceride metabolic process
autophagosome maturation
response to mitochondrial depolarisation
regulation of protein phosphorylation
positive regulation of autophagy
positive regulation of phosphatidylinositol 3-kinase activity
mitophagy in response to mitochondrial depolarization
mitophagy
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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