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Invitrogen
RAD52 Polyclonal Antibody
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RAD52 Antibody (PA5-119963) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded human liver carcinoma tissue using RAD52 Polyclonal Antibody (Product # PA5-119963). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the RAD52 antibody at a di... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-119963
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide within human RAD52 aa 331-380.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Control: Hela cell lysate, HL-60 cell lysate, human thyroid tissue, human colon carcinoma tissue, human liver carcinoma tissue, human spleen tissue, mouse liver tissue, rat spleen tissue.
Subcellular Location: Nucleus.
靶标信息
DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. Double-strand breaks are generally thought to be repaired via one of two distinct mechanisms: homologous recombination (HR) or non-homologous end joining (NHEJ). Homologous recombination requires the activity of the members of the RAD52 epistasis group, which includeRAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2. RAD52 can catalyze strand annealing reactions, and can form oligomers that attach to ends of single-stranded DNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA repair protein RAD52 homolog
基因别名: RAD52; Rad52yh
UniProt ID: (Mouse) P43352
Entrez Gene ID: (Rat) 297561, (Mouse) 19365
double-strand break repair via homologous recombination
DNA repair
DNA recombination
cellular response to oxidative stress
cellular response to DNA damage stimulus
DNA double-strand break processing involved in repair via single-strand annealing
double-strand break repair via single-strand annealing
regulation of nucleotide-excision repair
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