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Invitrogen
Phospho-IRF3 (Ser386) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-IRF3 (Ser386) Antibody (PA5-121307) in WB
Western blot analysis of Phospho-IRF3 (Ser386) in extracts of various cell lines. Samples were incubated with Phospho-IRF3 (Ser386) Polyclonal antibody (Product # PA5-121307) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. Lysates/protei... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-121307
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.59 mg/mL
Amount
159 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HeLa, NIH/3T3. The target is usually found in the following locations: Cytoplasm, Nucleus.
Immunogen sequence: ASSLE
Target Information
IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Interferon regulatory factor 3; IRF-3; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3
Gene Aliases: C920001K05Rik; IRF-3; IRF3
UniProt ID: (Mouse) P70671
Entrez Gene ID: (Rat) 292892, (Mouse) 54131
negative regulation of transcription from RNA polymerase II promoter
response to bacterium
lipopolysaccharide-mediated signaling pathway
response to lipopolysaccharide
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
type I interferon biosynthetic process
negative regulation of interferon-beta biosynthetic process
negative regulation of defense response to virus by host
positive regulation of cytokine secretion
defense response to virus
positive regulation of type I interferon-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to dsRNA
programmed necrotic cell death
immune system process
transcription, DNA-templated
regulation of transcription, DNA-templated
innate immune response
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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