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          • Primary Antibodies ›
          • IR/IGF1R Antibodies

          Invitrogen

          Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Polyclonal Antibody

          View all (8) IR/IGF1R antibodies

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          Cite Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Antibody (PA5-121332) in WB

          Western blot analysis of Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) in extracts of 293T cells. Samples were incubated with Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Polyclonal antibody (Product # PA5-121332) using a dilution of 1:1,000 or IGF1R antibody (Product # PA5-120037). Followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. 293T cells were trea... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Antibody in Western Blot (WB)
          Phospho-IR/IGF1R (Tyr1165, Tyr1166, Tyr1189, Tyr1190) Polyclonal Antibody

          Product Details

          PA5-121332

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RINSR (NP_0001992)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.42 mg/mL

          Amount

          142 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2914904

          Product Specific Information

          Positive test controls include: 293T.

          Immunogen sequence: YETDYY

          Target Information

          Insulin and Insulin-like Growth Factor receptors are transmembrane tyrosine kinase receptors that play critical roles in development, cell growth and metabolism. Insulin/Insulin-like growth factor-1 binding to the extracellular domain of IR and IGFR leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains contain a three tyrosine motif corresponding to Tyr1158, 1162 and 1163 (for the IR) and Tyr1131, 1135 and 1136 (for the IGF1R). Autophosphorylation of the tyrosine sites within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 or 1135), followed by phosphorylation at tyrosines 1158 (IGF-1R tyrosine 1131), 1163 (IGF-1R tyrosine 1136), resulting in the full activation of IR and IGF1R.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: CD220; CD221; IGF-I receptor; IGF-I Receptor beta; IGF1 Receptor; IGF1 Receptor beta; IGF1R beta; Insulin receptor; insulin receptor precursor; insulin receptor preproprotein; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IR; line 186; unnamed protein product

          View more View less

          Gene Aliases: 4932439J01Rik; A330103N21Rik; CD220; CD221; D630014A15Rik; D930020L01; HHF5; hyft; IGF-1R; IGF1R; IGFIR; IGFIRC; IGFR; Igfr1; INSR; IR; IR-A; IR-B; JTK13

          View more View less

          UniProt ID: (Human) P08069, (Human) P06213, (Rat) P24062, (Mouse) Q60751, (Mouse) P15208

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          Entrez Gene ID: (Human) 3480, (Human) 3643, (Rat) 25718, (Mouse) 16001, (Rat) 24954, (Mouse) 16337

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor-activated receptor activity insulin receptor binding structural molecule activity protein binding insulin-like growth factor binding ATP binding kinase activity transferase activity insulin-like growth factor I binding identical protein binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding protein transporter activity beta-amyloid binding insulin-like growth factor receptor binding GTP binding protein domain specific binding protein kinase activator activity insulin-like growth factor II binding cargo receptor activity macromolecular complex binding PTB domain binding G-protein alpha-subunit binding transferase activity, transferring phosphorus-containing groups receptor signaling protein tyrosine kinase activity protein kinase binding protein phosphatase binding lipoic acid binding protein complex binding 3-phosphoinositide-dependent protein kinase binding transmembrane signal receptor
          Process(es)
          immune response signal transduction transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of cell migration peptidyl-tyrosine autophosphorylation negative regulation of apoptotic process positive regulation of protein complex disassembly negative regulation of MAPK cascade positive regulation of MAPK cascade protein kinase B signaling transcytosis regulation of JNK cascade protein autophosphorylation insulin-like growth factor receptor signaling pathway positive regulation of protein kinase B signaling cellular response to glucose stimulus dendritic spine maintenance beta-amyloid clearance positive regulation of cold-induced thermogenesis cellular response to oxygen-containing compound cellular response to beta-amyloid positive regulation of receptor internalization heart morphogenesis regulation of glycogen biosynthetic process regulation of transcription, DNA-templated receptor-mediated endocytosis G-protein coupled receptor signaling pathway learning memory epidermis development male gonad development organ morphogenesis regulation of gene expression positive regulation of macromolecule biosynthetic process regulation of glucose metabolic process male sex determination adrenal gland development exocrine pancreas development receptor internalization cellular response to insulin stimulus glucose homeostasis positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of nitric oxide biosynthetic process positive regulation of glycogen biosynthetic process positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of embryonic development positive regulation of glucose import viral entry into host cell animal organ development positive regulation of developmental growth system development positive regulation of cellular component organization regulation of multicellular organismal process positive regulation of meiotic cell cycle positive regulation of respiratory burst cellular response to growth factor stimulus transport across blood-brain barrier neuron projection maintenance regulation of female gonad development protein phosphorylation axonogenesis brain development negative regulation of muscle cell apoptotic process establishment of cell polarity mammary gland development neuron projection development positive regulation of cytokinesis response to vitamin E negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of DNA replication phosphatidylinositol-mediated signaling positive regulation of DNA metabolic process protein tetramerization protein heterooligomerization inactivation of MAPKK activity negative regulation of protein kinase B signaling prostate gland epithelium morphogenesis positive regulation of steroid hormone biosynthetic process phosphorylation activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation response to hormone response to glucose response to manganese ion regulation of hydrogen peroxide metabolic process positive regulation of glycoprotein biosynthetic process negative regulation of gene expression response to activity peptidyl-tyrosine phosphorylation transformation of host cell by virus signal transduction by protein phosphorylation response to nutrient levels activation of protein kinase activity activation of protein kinase B activity response to estradiol negative regulation of transporter activity response to insulin response to vitamin D response to testosterone response to tumor necrosis factor positive regulation of phosphorylation fat cell differentiation response to ethanol protein heterotetramerization response to glucocorticoid intracellular signal transduction
          It has to be done as per old AB suggested Products section.

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