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Invitrogen
Troponin I Polyclonal Antibody
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FIGURE: 1 / 3
Troponin I Antibody (PA5-121540) in WB
Western blot analysis of Troponin I in Rat Skeletal muscle lysate using Troponin I Polyclonal Antibody (Product # PA5-121540) at a dilution of 1 µg/mL. Secondary antibody used is HRP-Goat anti-Rabbit IgG polyclonal antibody at a dilution of 0.2 µg/mL.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-121540
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Met1-Asp103 linked with Leu142-Gln187 (Accession P19237), with N-terminal His Tag
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cardiac troponin I; cTnI; DKFZp451O223; fast skeletal TnI; fsTnI; slow skeletal TnI; ssTnI; Tpn1; troponin 1, type 3; Troponin I, cardiac muscle; troponin I, skeletal, slow 1; Troponin I, slow isoform; Troponin I, slow skeletal muscle; Troponin I, slow-twitch isoform
Gene Aliases: 2700018B22Rik; AI747285; Ctni; ssTnI; Tn1; TnI; TNNC1; TNNI1; TNNI3
UniProt ID: (Mouse) Q9WUZ5, (Rat) P13413, (Rat) P23693, (Mouse) P48787
Entrez Gene ID: (Mouse) 21952, (Rat) 29388, (Rat) 29248, (Mouse) 21954
regulation of muscle contraction
transition between fast and slow fiber
ventricular cardiac muscle tissue morphogenesis
vasculogenesis
regulation of systemic arterial blood pressure by ischemic conditions
cellular calcium ion homeostasis
regulation of smooth muscle contraction
striated muscle contraction
heart development
negative regulation of ATPase activity
heart contraction
cardiac muscle contraction
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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