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Phospho-HGK (Ser629) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-HGK (Ser629) Antibody (PA5-12858) in DB
Dot blot analysis of Phospho-HGK (Ser629). Samples were incubated with Phospho-HGK (Ser629) polyclonal antibody (Product # PA5-12858) using a dilution of 0.5 µg/mL. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed on nitrocellulose membrane.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-12858
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S629 of human MAP4K4
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
200 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
MAP4K4 (HGK) is a serine/threonine kinase which plays an important role in stress-activated MAPK core signaling pathways, the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory protein Li 31; FLJ10410; FLJ20373; FLJ90111; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; nck-interacting kinase; Ste20 group protein kinase HGK; tumor-associated splice form; unnamed protein product
Gene Aliases: FLH21957; HEL-S-31; HGK; MEKKK4; NIK
UniProt ID: (Human) O95819
Entrez Gene ID: (Human) 9448
MAPK cascade
negative regulation of cell-matrix adhesion
protein phosphorylation
positive regulation of cell migration
positive regulation of ARF protein signal transduction
positive regulation of hippo signaling
intracellular signal transduction
negative regulation of apoptotic process
regulation of MAPK cascade
regulation of JNK cascade
neuron projection morphogenesis
positive regulation of keratinocyte migration
positive regulation of focal adhesion assembly
positive regulation of focal adhesion disassembly
positive regulation of intracellular signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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