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Invitrogen
AKR1D1 Polyclonal Antibody
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FIGURE: 1 / 5
AKR1D1 Antibody (PA5-143935) in ICC/IF
Immunocytochemistry analysis of AKR1D1 in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in AKR1D1 Polyclonal antibody (Product # PA5-143935) using a dilution of 4 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-143935
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human AKR1D1 (KGACATSVKVAIDTGYRHIDGAYIYQNEHE).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, mouse liver tissue, human caco-2 whole cell. IHC: human liver cancer tissue. ICC/IF: U2OS cell. Flow: CACO-2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-oxo-5-beta-steroid 4-dehydrogenase; 37 kda protein; aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase); delta(4)-3-ketosteroid 5-beta-reductase; delta(4)-3-oxosteroid 5-beta-reductase; steroid-5-beta-reductase, beta polypeptide 1 (3-oxo-5 beta-steroid delta 4-dehydrogenase beta 1); unnamed protein product
Gene Aliases: 3o5bred; CBAS2; SRD5B1
UniProt ID: (Human) P51857, (Mouse) Q8VCX1, (Rat) P31210
Entrez Gene ID: (Human) 6718, (Mouse) 208665, (Rat) 192242
alditol:NADP+ 1-oxidoreductase activity
steroid binding
protein binding
alcohol dehydrogenase (NADP+) activity
steroid dehydrogenase activity
oxidoreductase activity
ketosteroid monooxygenase activity
delta4-3-oxosteroid 5beta-reductase activity
17-beta-hydroxysteroid dehydrogenase (NADP+) activity
3-oxo-5-beta-steroid 4-dehydrogenase activity
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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