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          • Primary Antibodies ›
          • SRC Antibodies

          Invitrogen

          SRC Polyclonal Antibody

          Advanced Verification
          1 Published Figure
          1 Reference
          View all (61) SRC antibodies

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          Cite SRC Polyclonal Antibody

          Additional Information:

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          • Antibody Testing Data (1)
          • Published Figures (1)
          • Advanced Verification (1)
          SRC Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          SRC Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          SRC Antibody (PA5-17717) in WB

          Western blot was performed using Anti-SRC Polyclonal Antibody (Product # PA5-17717) and 58, 60 kDa bands corresponding to Proto-oncogene tyrosine-protein kinase Src were observed across models tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), MCF7 (Lane 3), PC-3 (Lane 4), Raji (Lane 5), Mouse Urinary Bladder (Lane 6), Mouse Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          SRC Antibody in Western Blot (WB)
          SRC Antibody in Western Blot (WB)
          SRC Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          SRC Polyclonal Antibody

          Product Details

          PA5-17717

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Chicken, Human, Mouse, Rat

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues surrounding Tyr530 of human Src.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          157 µg/mL

          Amount

          15.7 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10980118

          Product Specific Information

          It is not recommended to aliquot this antibody.

          Target Information

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: c-Src; c-src protein, C terminus; EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; non-tyrosine protein kinase; ORF; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-scr; pp60c-src; pp60src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; Rous sarcoma oncogene; RP5-823N20.1; src downstream region; tyrosine kinase pp60c-src; tyrosine protein kinase c-src; tyrosine protein kinase pp60-c-src; tyrosine-protein kinase SRC-1; tyrosine-specific protein kinase; Unknown; unnamed protein product; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma viral oncogene

          View more View less

          Gene Aliases: ASV; c-SRC; p60-Src; PP60C-SCR; pp60c-src; SDR; SRC1; THC6

          View more View less

          UniProt ID: (Chicken) P00523, (Human) P12931, (Mouse) P05480, (Rat) Q9WUD9

          View more View less

          Entrez Gene ID: (Chicken) 396442, (Human) 6714, (Mouse) 20779, (Rat) 83805

          View more View less

          Function(s)
          protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding protein phosphatase binding heme binding growth factor receptor binding connexin binding protein kinase activity integrin binding phospholipase activator activity kinase activity enzyme binding protein domain specific binding protein tyrosine kinase activator activity receptor activator activity ionotropic glutamate receptor binding SH2 domain binding phospholipase binding ion channel binding cadherin binding ephrin receptor binding ATPase binding phosphoprotein binding BMP receptor binding scaffold protein binding protein kinase C binding insulin receptor binding protein kinase binding estrogen receptor binding macromolecular complex binding cell adhesion molecule binding transmembrane signal receptor scaffold/adaptor protein
          Process(es)
          cell cycle cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway cell differentiation osteoclast development peptidyl-tyrosine autophosphorylation regulation of cell proliferation innate immune response bone resorption progesterone receptor signaling pathway regulation of cell cycle negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway negative regulation of transcription from RNA polymerase II promoter stimulatory C-type lectin receptor signaling pathway negative regulation of inflammatory response to antigenic stimulus signal transduction signal complex assembly transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway lactation regulation of epithelial cell migration positive regulation of epithelial cell migration macroautophagy peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion platelet activation osteoclast differentiation T cell costimulation negative regulation of protein complex assembly protein destabilization negative regulation of telomere maintenance positive regulation of integrin activation regulation of toll-like receptor 3 signaling pathway cellular response to reactive oxygen species positive regulation of Rac protein signal transduction positive regulation of dephosphorylation negative regulation of hippo signaling intracellular signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway angiotensin-activated signaling pathway vasodilation negative regulation of apoptotic process regulation of vascular permeability stress fiber assembly regulation of bone resorption positive regulation of Notch signaling pathway positive regulation of glycolytic process viral entry into host cell vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway focal adhesion assembly leukocyte migration positive regulation of small GTPase mediated signal transduction negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization intestinal epithelial cell development interleukin-6-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to peptide hormone stimulus cellular response to progesterone stimulus regulation of heart rate by cardiac conduction negative regulation of neutrophil activation positive regulation of TORC1 signaling positive regulation of lamellipodium morphogenesis regulation of early endosome to late endosome transport negative regulation of anoikis regulation of caveolin-mediated endocytosis primary ovarian follicle growth positive regulation of cytokine production protein phosphorylation gamete generation positive regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression positive regulation of glucose metabolic process positive regulation of protein processing positive regulation of smooth muscle cell migration cell migration protein deubiquitination positive regulation of cell migration forebrain development cellular response to nutrient levels regulation of intracellular estrogen receptor signaling pathway toll-like receptor 3 signaling pathway substrate adhesion-dependent cell spreading cellular response to platelet-derived growth factor stimulus TORC1 signaling odontogenesis positive regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of MAP kinase activity transcytosis positive regulation of bone resorption positive regulation of cell adhesion positive regulation of cell size positive regulation of Ras protein signal transduction positive regulation of insulin receptor signaling pathway neurotrophin TRK receptor signaling pathway oogenesis positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein transport uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly cellular response to hydrogen peroxide cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of podosome assembly DNA biosynthetic process positive regulation of canonical Wnt signaling pathway positive regulation of wound healing positive regulation of protein localization to nucleus positive regulation of intracellular signal transduction positive regulation of cellular protein localization negative regulation of TORC1 signaling cellular response to angiotensin positive regulation of vascular smooth muscle cell proliferation positive regulation of neural precursor cell proliferation positive regulation of male germ cell proliferation positive regulation of ovarian follicle development positive regulation of platelet-derived growth factor receptor-beta signaling pathway spermatogenesis learning or memory response to xenobiotic stimulus response to mechanical stimulus response to acidic pH skeletal muscle cell proliferation response to nutrient levels cellular response to insulin stimulus response to ATP adherens junction organization response to hydrogen peroxide platelet-derived growth factor receptor signaling pathway behavioral response to cocaine response to mineralocorticoid myoblast proliferation response to electrical stimulus response to fatty acid cellular response to lipopolysaccharide cellular response to fatty acid cellular response to hypoxia cell-cell adhesion positive regulation of outer hair cell apoptotic process cellular response to prolactin
          It has to be done as per old AB suggested Products section.

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