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Invitrogen
Blimp-1 Polyclonal Antibody
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FIGURE: 1 / 4
Blimp-1 Antibody (PA5-18110) in ICC/IF
Immunocytochemistry analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Samples were incubated with Blimp-1 polyclonal antibody (Product # PA5-18110) using a dilution of 10 µg/mL for 1 hr followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-18110
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (KVKQETVEPMDP) corresponding to the C-terminus amino acids of PRDM1 (aa 814-825).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-interferon gene positive regulatory domain I-binding factor; BLIMP 1; BLIMP-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; RP1-134E15.1
Gene Aliases: BLIMP1; PRDM1
Entrez Gene ID: (Human) 639
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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