Invitrogen

AMPD2 Polyclonal Antibody

2个已发表图片

3 篇参考文献

查看其他6个AMPD2抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (3)
已发表图片 (2)

AMPD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AMPD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 5

AMPD2 Antibody (PA5-26127) in IHC (P)

Immunohistochemistry analysis of AMPD2 in formalin-fixed and paraffin-embedded human skeletal muscle. Samples were incubated with AMPD2 polyclonal antibody (Product # PA5-26127) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AMPD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AMPD2 Antibody in Western Blot (WB)

AMPD2 Antibody in Flow Cytometry (Flow)

AMPD2 Antibody in Western Blot (WB)

AMPD2 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

AMPD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of AMPD2 in formalin-fixed and paraffin-embedded human skeletal muscle. Samples were incubated with AMPD2 polyclonal antibody (Product # PA5-26127) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AMPD2 Antibody in Western Blot (WB) — Western blot analysis of AMPD2 in K562 cell line lysates. Samples were incubated with AMPD2 polyclonal antibody (Product # PA5-26127). Lysates: 35 µg/lane. AMPD2 (arrow).

AMPD2 Antibody in Flow Cytometry (Flow) — Flow cytometry of AMPD2 in K562 cells (bottom histogram). Samples were incubated with AMPD2 polyclonal antibody (Product # PA5-26127) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).

AMPD2 Antibody in Western Blot (WB) — Verification of anti-GR (5E4) antibody specificity. ( A ) Western blot analysis of GR pulled down from HEK293 membrane fractions by immunoprecipitation using the anti-GR (5E4) antibody (IP 5E4). An amount of 20 uL of membrane fraction protein (lysate) were analyzed in parallel. Protein detection was achieved by adding the anti-GR (5E4) antibody followed by an HRP-conjugated anti-mouse IgG antibody as a secondary reagent. ( B ) Immunoprecipitation from HEK293 membrane fractions was performed using the anti-GR (5E4) antibody (IP 5E4) and mouse IgG1 as a corresponding isotype control (IP IgG1). For mass spectrometric analysis the protein content was visualized by Pierce Coomassie Brilliant Blue G-250 Dye after SDS-PAGE, and the indicated area of interest was extracted for analysis. ( C ) Mass spectrometric analyses of pull-down samples obtained by immunoprecipitation from HEK293, Jurkat, and THP-1 whole cell lysates using the anti-GR (5E4) antibody (AB) and mouse IgG1 as corresponding isotype control (Control). Differential protein abundance compared to isotype control was determined using a two-sample Student's t test and black circles represent significance with an FDR cut-off of 5%. ( D ) Western blot analysis of GR pulled down from HEK293 whole cell lysates by immunoprecipitation using anti-GR (5E4) antibody Lot #1 (provided by Timea Berki []) and Lot #2 (Bio-Rad, Cat# MCA2469, RRID:AB_10844347). The protein content was visualized by incubation with anti-GR (5E4) antibody fo

AMPD2 Antibody in Western Blot (WB) — Re-evaluation of anti-GR (5E4) antibody specificity. ( A ) Comparison of target proteins by western blot analysis. Pull-down samples from HEK293 whole cell lysates were obtained by immunoprecipitation using the following antibodies: anti-GR (5E4), anti-GR (G-5), anti-AMPD2 (QQ13), and anti-TRIM28 (Cat# PA5-27648). The protein content was visualized by incubation with primary antibodies directed against GR (5E4, biotinylated), GR (G-5, biotinylated), AMPD2 (PA5-26127, biotinylated), and TRIM28 (Cat# PA5-27648) as indicated, followed by HRP-conjugated streptavidin and anti-rabbit IgG antibody as secondary reagents. ( B ) Mass spectrometric analysis of pull-down samples obtained by IP from HEK293 whole cell lysates using the anti-GR antibody, 5E4, with and without prior two-hour incubation with APTEK-26 peptide. Bar graphs show fold change of peptide incubation to without peptide incubation. ( C ) Amino acid sequences of the newly identified anti-GR (5E4) target proteins, AMPD2 (UniProt ID: Q01433) and TRIM28 (UniProt ID: Q13263), were blasted against the APTEK-26 peptide using NCBI Protein BLAST ( https://blast.ncbi.nlm.nih.gov/Blast.cgi , accessed on 14 November 2020) [].

Immunogen image

AMPD2 Polyclonal Antibody

Immunogen Image AMPD2 Polyclonal Antibody

产品信息

PA5-26127

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:100

-

流式细胞分析 (Flow)

1:10-1:50

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免疫沉淀 (IP)

-

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 187-217 amino acids from the central region of human AMPD2

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

400 µg

纯化类型

Ammonium sulfate precipitation, Size-exclusion - Dialysis

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Adenosine monophosphate deaminase-2 (EC 3.5.4.6) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: adenosine monophosphate deaminase 2 (isoform L); AMP deaminase 2; AMP deaminase isoform L; unnamed protein product

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基因别名: AMPD; AMPD2; PCH9; SPG63

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Entrez Gene ID: (Human) 271

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AMP deaminase activity protein binding deaminase activity identical protein binding

IMP biosynthetic process purine ribonucleoside monophosphate biosynthetic process GMP salvage IMP salvage AMP metabolic process ATP metabolic process GTP metabolic process cyclic purine nucleotide metabolic process energy homeostasis purine ribonucleotide salvage