Search

  • Contact Us
  • Quick Order

Invitrogen

COX2 Polyclonal Antibody

Advanced Verification
1 Published Figure
2 References
View all (44) COX2 antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
COX2 Polyclonal Antibody

Product Details

PA5-27238

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

ELISA (ELISA)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX2. The exact sequence is proprietary.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.97 mg/mL

Amount

197 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544714

Product Specific Information

Recommended positive controls: THP-1 (100 ng/mL PMA treatment for 16 hr and 1000 ng/mL LPS treatment for 24 hr), RAW264.7 (0.1 µg/mL PMA treatment for 16 hr and 1 µg/mL LPS treatment for 24 hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: COX-2; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2 splice variant; cyclooxygenase 2b; Cyclooxygenase-2; glucocorticoid-regulated inflammatory cyclooxygenase; macrophage activation-associated marker protein P71/73; PGH synthase 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; prostaglandin synthase; prostaglandin-endoperoxide synthase; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); unnamed protein product

View more View less

Gene Aliases: COX-2; COX2; GRIPGHS; hCox-2; PES-2; PGG/HS; PGHS-2; Pghs2; PHS II; PHS-2; PTGS2; TIS10

View more View less

Entrez Gene ID: (Human) 5743, (Mouse) 19225, (Rat) 29527

View more View less

Function(s)
peroxidase activity prostaglandin-endoperoxide synthase activity protein binding oxidoreductase activity, acting on single donors with incorporation of molecular oxygen oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen enzyme binding heme binding protein homodimerization activity
Process(es)
prostaglandin biosynthetic process angiogenesis response to oxidative stress embryo implantation learning memory blood circulation regulation of blood pressure positive regulation of cell proliferation negative regulation of cell proliferation response to nematode response to selenium ion positive regulation of vascular endothelial growth factor production cyclooxygenase pathway keratinocyte differentiation bone mineralization positive regulation of prostaglandin biosynthetic process positive regulation of fever generation positive regulation of synaptic plasticity negative regulation of synaptic transmission, dopaminergic prostaglandin secretion response to lipopolysaccharide response to cytokine maintenance of blood-brain barrier regulation of cell proliferation positive regulation of protein import into nucleus positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of nitric oxide biosynthetic process negative regulation of cell cycle positive regulation of vasoconstriction negative regulation of smooth muscle contraction positive regulation of smooth muscle contraction prostanoid biosynthetic process decidualization positive regulation of smooth muscle cell proliferation brown fat cell differentiation negative regulation of calcium ion transport positive regulation of synaptic transmission, glutamatergic cellular response to hypoxia cellular response to non-ionic osmotic stress cellular response to fluid shear stress positive regulation of transforming growth factor beta production positive regulation of cell migration involved in sprouting angiogenesis positive regulation of fibroblast growth factor production positive regulation of brown fat cell differentiation positive regulation of platelet-derived growth factor production cellular oxidant detoxification positive regulation of hippocampal neuron apoptotic process regulation of neuroinflammatory response negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress positive regulation of glutamate secretion, neurotransmission lipoxygenase pathway long-chain fatty acid biosynthetic process regulation of inflammatory response response to xenobiotic stimulus response to fructose response to manganese ion response to estradiol response to vitamin D cellular response to heat response to tumor necrosis factor hair cycle response to glucocorticoid response to fatty acid cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to lead ion cellular response to ATP cellular response to homocysteine response to angiotensin
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support