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Invitrogen
V-ATPase E1 Polyclonal Antibody
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FIGURE: 1 / 11
V-ATPase E1 Antibody (PA5-29899) in ICC/IF
Immunofluorescence analysis of V-ATPase E1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-V-ATPase E1 Rabbit Polyclonal Antibody (Product # PA5-29899) at 1:200 dilution in 0.1% BSA, incubated at ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-29899
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATP6V1E1. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.73 mg/mL
Amount
173 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: A549, H1299, HCT116, mouse brain, rat brain.
Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (82%), Chicken (87%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATPase, H+ transporting lysosomal (vacuolar proton pump), 32 kDa; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E; ATPase, H+ transporting, V1 subunit E; H(+)-ATPase E-like protein; H+ ATPase subunit E; lysosomal 31kDa; p31; V-ATPase 31 kDa subunit; V-ATPase subunit E; V-ATPase subunit E 1; V-type proton ATPase subunit E 1; vacuolar H(+)-ATPase, E subunit; vacuolar H+ ATPase E1; Vacuolar proton pump subunit E 1; VATPase, H+ transporting, lysosomal V1 subunit E1
Gene Aliases: 2410029D23Rik; ATP6E; ATP6E2; Atp6v1e; ATP6V1E1; D6Ertd385e; E2; P31; Vma4
UniProt ID: (Mouse) P50518, (Rat) Q6PCU2
Entrez Gene ID: (Mouse) 11973, (Rat) 297566
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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