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Invitrogen

Phospho-PAK1/PAK2 (Thr423, Thr402) Polyclonal Antibody

View all (4) PAK1/PAK2 antibodies

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Phospho-PAK1/PAK2 (Thr423, Thr402) Polyclonal Antibody

Product Details

PA5-36888

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Threonine 423
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2553802

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 60-70 kDa.

Purity is >95% by SDS-PAGE.

Target Information

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: activated protein kinase alpha; Alpha-PAK; Beta-PAK; CDC42/RAC effector kinase PAK-A; gamma pak; Gamma-PAK; Oligophrenin-3; OPHN3; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 1; p21-activated kinase 2; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p58; p65-PAK; p68-PAK; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK3; PAK65; protein kinase; Protein kinase MUK2; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; STE20 homolog, yeast; unnamed protein product

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Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; alpha-PAK; AW045634; D16Ertd269e; gamma-PAK; IDDMSSD; KNO2; mKIAA4182; p65-PAK; PAK-1; PAK-2; PAK1; PAK2; PAK65; Paka; PAKalpha; PAKgamma

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UniProt ID: (Human) Q13153, (Human) Q13177, (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303

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Entrez Gene ID: (Human) 5058, (Human) 5062, (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432

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Function(s)
nucleotide binding catalytic activity protein kinase activity protein serine/threonine kinase activity protein binding collagen binding ATP binding kinase activity transferase activity protein kinase binding small GTPase binding identical protein binding gamma-tubulin binding protein serine kinase activity protein tyrosine kinase activator activity cadherin binding transferase activity, transferring phosphorus-containing groups Rac GTPase binding MAP kinase kinase kinase activity receptor signaling protein serine/threonine kinase activity non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade response to hypoxia stimulatory C-type lectin receptor signaling pathway chromatin remodeling exocytosis apoptotic process cellular response to DNA damage stimulus transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation cellular response to starvation positive regulation of fibroblast migration cell migration cerebellum development establishment of cell polarity actin cytoskeleton organization positive regulation of cell migration positive regulation of microtubule polymerization cellular response to insulin stimulus regulation of actin cytoskeleton organization positive regulation of intracellular estrogen receptor signaling pathway intracellular signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis wound healing regulation of MAPK cascade positive regulation of axon extension positive regulation of insulin receptor signaling pathway hepatocyte growth factor receptor signaling pathway ephrin receptor signaling pathway branching morphogenesis of an epithelial tube neuron projection morphogenesis regulation of axonogenesis positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition negative regulation of cell growth involved in cardiac muscle cell development positive regulation of microtubule nucleation positive regulation of protein targeting to membrane regulation of actin filament organization protein localization to cytoplasmic stress granule positive regulation of vascular smooth muscle cell proliferation positive regulation of vascular associated smooth muscle cell migration cardiac muscle hypertrophy protein phosphorylation negative regulation of protein kinase activity signal transduction adherens junction assembly negative regulation of apoptotic process protein autophosphorylation vascular endothelial growth factor receptor signaling pathway regulation of cytoskeleton organization negative regulation of stress fiber assembly protein maturation dendritic spine development bicellular tight junction assembly cellular response to transforming growth factor beta stimulus execution phase of apoptosis protein localization to cell-cell junction positive regulation of execution phase of apoptosis positive regulation of extrinsic apoptotic signaling pathway positive regulation of protein phosphorylation Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression phosphorylation dendrite development amygdala development signal transduction by protein phosphorylation stress-activated protein kinase signaling cascade actin cytoskeleton reorganization activation of protein kinase activity positive regulation of peptidyl-serine phosphorylation regulation of apoptotic process receptor clustering positive regulation of JUN kinase activity gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission cellular response to organic cyclic compound observational learning regulation of long-term synaptic potentiation activation of MAPKK activity mitotic cell cycle small GTPase mediated signal transduction axonogenesis peptidyl-serine phosphorylation regulation of growth positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein tyrosine kinase activity negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
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