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Invitrogen
Myosin 4 Polyclonal Antibody
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FIGURE: 1 / 4
Myosin 4 Antibody (PA5-50065) in ICC/IF
Immunofluorescence analysis of MYH4 was performed using 70% confluent log phase C2C12 cells and C2C12 differentiated Myotubes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with Myosin 4 Polyclonal Antibody (Product # PA5-50065) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-50065
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthesized peptide derived from C-terminal of human MYH4.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects endogenous levels of total Myosin-4 protein.
Target Information
Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 71; myHC-IIx/d; myosin heavy chain 2b; myosin heavy chain 2x; myosin heavy chain IIb; myosin heavy chain IIx/d; myosin heavy chain, skeletal muscle, adult 1; myosin heavy chain, skeletal muscle, fetal; myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 1, skeletal muscle, adult; myosin, heavy polypeptide 4, skeletal muscle; unnamed protein product
Gene Aliases: HEL71; MYH2B; MYHa; MyHC-2B; MyHC-2x; MyHC-2X/D; MyHC-IIb; MYHSA1
UniProt ID: (Human) P12882, (Human) Q9Y623
Entrez Gene ID: (Human) 4619, (Human) 4622
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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