Search
Invitrogen
Phospho-SIK2 (Thr175, Thr221) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:
FIGURE: 1 / 1
Phospho-SIK2 (Thr175, Thr221) Antibody (PA5-64607) in WB
Western Blot analysis of Phospho-SIK2 (Thr175, Thr163) was performed using lysates of (1) HeLa and (2) HeLa treated with 100 ng/mL LPS for 20 min. The membrane was probed with Phospho-SIK2 (Thr175, Thr163) Polyclonal Antibody (Product # PA5-64607) using a dilution of 1:1,000.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-64607
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human QIK/QSK (Phospho-Thr182/Thr175/Thr221).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Phospho-SIK2 (Thr175, Thr163) Polyclonal Antibody detects endogenous levels of SIK2 only when phosphorylated at Thr175, Thr163.
Target Information
SNF1LK2 (QIK) is a serine/threonine kinase and is a salt-inducible kinase that belongs to the AMP-activated protein kinase family. SNF1LK2 negatively regulates CRE-binding protein (CREB) activity by phosphorylating the CREB-specific coactivator transducer of regulated CREB activity (TORC). SIK2 is thought to be part of a signaling cascade that regulates the expression and activity of the insulin-induced genes PGC-1'alpha and UCP-1 in brown adipocytes, impairment of which has been implicated in obesity and insulin resistance in human and animal models. SIK2 has also been reported as a key regulator for neuronal survival after ischemia, suppressing CREB-mediated gene expression after oxygen-glucose deprivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: qin-induced kinase; salt induceable kinase 2; salt-inducible protein kinase 2; salt-inducible serine/threonine kinase 2; serine/ threonine protein kinase; serine/threonine-protein kinase SNF1-like kinase 2; SIK-2; SNF1-like kinase 2; unnamed protein product
Gene Aliases: G630080D20Rik; LOH11CR1I; QIK; RGD1559698; SIK-2; SNF1LK2
UniProt ID: (Human) Q9H0K1, (Mouse) Q8CFH6
Entrez Gene ID: (Human) 23235, (Rat) 315649, (Mouse) 235344
protein phosphorylation
insulin receptor signaling pathway
intracellular signal transduction
regulation of insulin receptor signaling pathway
protein autophosphorylation
negative regulation of transcription from RNA polymerase II promoter by glucose
positive regulation of ERAD pathway
lipid biosynthetic process
ERAD pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support