Invitrogen

SMO Polyclonal Antibody

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问题与答案

问题与答案

问题与答案

Additional Information:

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SMO Antibody in Neutralization (Neu)

SMO Antibody in Neutralization (Neu)

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SMO Antibody (PA5-72395) in Neu

Cell reports 2024 - Figure 1. shRNA-mediated CerS4 knockdown activates Shh and TGFBR1 signaling (A) Shh protein abundance in SCR or CerS4 shRNA-transfected 4T1 cells was detected in western blots. Data quantification is shown in right panel ( n = 3). (B) ELISA detected shh protein in the cell-culture supernatant from SCR or CerS4 shRNA-transfected 4T1 cells ( n = 4). (C) Representative images of migration assay measured in fib... View More

SMO Antibody in Neutralization (Neu)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SMO Antibody in Neutralization (Neu) — Figure 1. shRNA-mediated CerS4 knockdown activates Shh and TGFBR1 signaling (A) Shh protein abundance in SCR or CerS4 shRNA-transfected 4T1 cells was detected in western blots. Data quantification is shown in right panel ( n = 3). (B) ELISA detected shh protein in the cell-culture supernatant from SCR or CerS4 shRNA-transfected 4T1 cells ( n = 4). (C) Representative images of migration assay measured in fibronectin-coated Boyden chambers ( n = 3). Scale bars, 10 mum. (D) Interaction of Shh and PTCH1 was detected by a co-immunoprecipitation with a Shh-targeting antibody and blotting for Shh and PTCH1 in 4T1 cells stably transfected with SCR or CerS4 shRNA ( n = 2). Data quantification is shown in right panel. (E) Effect of exogenous shh ligand (100-200 mug/mL) on cellular migration in 4T1 cells stably expressing SCR or CerS4 shRNA ( n = 3). (F) Effect of a neutralizing SMO and pSMO targeting antibody on cellular migration in 4T1 cells stably expressing SCR or CerS4 shRNA. Data quantification isshown in right panel ( n = 3). Scale bars, 10 mum. (G) Effect of exogenous shh ligand (200 mug/mL) on cellular migration in 4T1 cells stably expressing SCR or CerS4 shRNA with or without neutralizing pSMO antibody treatment ( n = 2). (H) TGFBR1 protein abundance in SCR or CerS4 shRNA-transfected 4T1 cells was detected by western blot. Data quantification is shown in lower panel ( n = 4). (I) Interaction of pSmo and TGFBR1 proteins was measured using a PLA in 4T1 cells stably expressing S

Immunogen image

SMO Polyclonal Antibody

Immunogen Image SMO Polyclonal Antibody

产品信息

PA5-72395

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

-

中和实验 (Neu)

-

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

region 85-116 aa

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 aa, seven transmembrane G protein-coupled receptors with 4 glycosylation sites and a putative extracellular amino terminus. Smoothened activated by b-Arrestin 2 and GRK2 acts as a receptor for Shh, the Sonic hedgehog gene product, and is a positive regulator of hedgehog signaling pathway along with the smoothened inhibitor patched protein (PTCH) and regulates vertebrate developmental processes, cellular proliferation and differentiation by activating transcription factor Gli. Smoothened mutants are known to cause sporadic basal cell carcinoma of the skin. It is known to express widely in heart and brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: bent body; HGNC:11119; Protein Gx; Protein SMOH; Protein smoothened; smoothened homolog; smoothened, frizzled family receptor

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基因别名: bnb; E130215L21Rik; SMO; SMOH; smoothened

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UniProt ID: (Mouse) P56726, (Rat) P97698

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Entrez Gene ID: (Mouse) 319757, (Rat) 25273

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signal transducer activity transmembrane signaling receptor activity G-protein coupled receptor activity patched binding drug binding Wnt-protein binding molecular_function Wnt-activated receptor activity transmembrane signal receptor

negative regulation of transcription from RNA polymerase II promoter ossification vasculogenesis osteoblast differentiation in utero embryonic development cell fate specification neural crest cell migration heart looping positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation heart morphogenesis determination of left/right asymmetry in lateral mesoderm type B pancreatic cell development signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway smoothened signaling pathway positive regulation of hh target transcription factor activity multicellular organism development determination of left/right symmetry ventral midline determination pattern specification process central nervous system development midgut development positive regulation of cell proliferation anterior/posterior pattern specification dorsal/ventral pattern formation regulation of gene expression positive regulation of gene expression negative regulation of gene expression dentate gyrus development cerebellar cortex morphogenesis thalamus development dorsal/ventral neural tube patterning smoothened signaling pathway involved in ventral spinal cord patterning smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation central nervous system neuron differentiation cerebral cortex development negative regulation of epithelial cell differentiation hair follicle morphogenesis protein localization to nucleus multicellular organism growth positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth negative regulation of apoptotic process negative regulation of DNA binding positive regulation of smoothened signaling pathway positive regulation of transcription from RNA polymerase II promoter astrocyte activation cell development digestive tract development embryonic organ development developmental growth skeletal muscle fiber development axon extension involved in axon guidance forebrain morphogenesis homeostasis of number of cells within a tissue positive regulation of epithelial cell proliferation protein stabilization myoblast migration negative regulation of hair follicle development detection of cell density by contact stimulus involved in contact inhibition atrial septum morphogenesis mammary gland epithelial cell differentiation epithelial-mesenchymal cell signaling somite development pancreas morphogenesis left/right axis specification mesenchymal to epithelial transition involved in metanephric renal vesicle formation positive regulation of branching involved in ureteric bud morphogenesis regulation of stem cell population maintenance regulation of heart morphogenesis spermatogenesis response to wounding response to organic substance facial nerve development neuron projection regeneration osteoblast proliferation non-canonical Wnt signaling pathway canonical Wnt signaling pathway cellular response to cholesterol renal system development