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Invitrogen
APOBEC3G Polyclonal Antibody
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APOBEC3G Antibody (PA5-78801) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of APOBEC3G in A431 cells using APOBEC3G Polyclonal Antibody (Product # PA5-78801). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filt... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-78801
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human APOBEC3G recombinant protein (Position: E191-N384).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell lysates, human HEL whole cell lysates, human RT4 whole cell lysates, rat testis tissue lysates, rat PC-12 whole cell lysates, mouse testis tissue lysates, mouse RAW264.7 whole cell lysates. ICC/IF: A431 cell.
靶标信息
The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5-a which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A3G; APOBEC-related cytidine deaminase; APOBEC-related protein; APOBEC-related protein 9; apolipoprotein B editing complex 3; Apolipoprotein B mRNA-editing complex 3; ARP-9; Arp3; ASGP-R2; ASGPR2; CEM-15; CEM15; CLEC4H2; Deoxycytidine deaminase; DNA dC->dU-editing enzyme APOBEC-3; DNA dC->dU-editing enzyme APOBEC-3G; FLJ12740; HBXBP; HL-2; OTTHUMP00000028911; recovery from Friend virus 3
基因别名: Apobec3; APOBEC3G; Arp3; BC003314; Cem15; MDS019; Rfv3
UniProt ID: (Mouse) Q99J72
Entrez Gene ID: (Mouse) 80287
catalytic activity
GTP cyclohydrolase activity
protein binding
tRNA-specific adenosine deaminase activity
zinc ion binding
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
N-cyclopropylmelamine deaminase activity
N-cyclopropylammeline deaminase activity
N-cyclopropylammelide alkylamino hydrolase activity
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate deaminase activity
tRNA-specific adenosine-37 deaminase activity
archaeal-specific GTP cyclohydrolase activity
poly(A) RNA binding
metal ion binding
deoxycytidine deaminase activity
tRNA-specific adenosine-34 deaminase activity
RNA processing factor
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