Invitrogen

BIK Polyclonal Antibody

Name: BIK Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-78873
Availability: InStock

高级验证

查看其他15个BIK抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (9)
高级验证 (1)

BIK Antibody in Immunocytochemistry (ICC/IF)

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BIK Antibody (PA5-78873) in ICC/IF

Immunofluorescence analysis of BIK was performed using Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with BIK Polyclonal Antibody (Product # PA5-78873) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 f... View More

BIK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

BIK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of BIK was observed between Raji, Ramos and SH-SY5Y, where SH-SY5Y is reported to be negative for BIK expression using Anti-BIK Polyclonal Antibody (Product # PA5-78873) in Western Blot. {RE}

Immunogen image

Immunogen Image BIK Polyclonal Antibody

产品信息

PA5-78873

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

0.5-1 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Bik recombinant protein (Position: M1-R123).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2745989

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: Rat Spleen Tissue, Rat Gaster Tissue, Rat Intestine Tissue, MCF-7 whole cell, A549 whole cell, SKOV whole cell. IHC: Mouse Spleen tissue, Rat Spleen tissue, Human Thyroid Cancer tissue. Flow: MCF-7 cell, THP-1 cell, A431 cell.

靶标信息

The Bik protein is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programmed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK.

仅用于科研。不用于诊断过程。未经明确授权不得转售。