Invitrogen

Menin Polyclonal Antibody

Name: Menin Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79663
Availability: InStock

高级验证

1 篇参考文献

查看其他22个Menin抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (5)
高级验证 (1)

Menin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

Menin Antibody (PA5-79663) in ICC/IF

Immunofluorescence analysis of MEN1 was performed using 70% confluent log phase HEK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MEN1 Polyclonal Antibody (Product # PA5-79663) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Produc... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Menin Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK 293 cells were transfected with MEN1 siRNA and decrease in signal intensity was observed in western blot application using MEN1 Polyclonal Antibody (Product # PA5-79663) {KD}

Immunogen image

Immunogen Image Menin Polyclonal Antibody

产品信息

PA5-79663

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Menin recombinant protein (Position: P301-L615).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

0.9mg NaCl, 0.2mg Na2PO4, 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746778

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: HELA whole cell, 293T whole cell, SMMC whole cell, HEPA whole cell, COLO320 whole cell.

靶标信息

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.

仅用于科研。不用于诊断过程。未经明确授权不得转售。