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Invitrogen
TLR8 Polyclonal Antibody
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FIGURE: 1 / 3
TLR8 Antibody (PA5-80137) in WB
Western Blot was performed using Anti-TLR8 Polyclonal Antibody (Product # PA5-80137) and a 150 kDa band corresponding to full length Toll-like receptor 8 was observed in ssRNA40 stimulated THP-1 cells but not in B-cells, which are reported negative for TLR8. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), IFN-gamma stimulated THP-1 (Lane 2), IFN-gamma stimulated THP-1 cells treated with ssRNA40 (Lane 3), Ramos (Lane 4), and Raji (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved pro... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-80137
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat TLR8 (1013-1029aa ENDSRYDNLYIDSIRQY).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue.
Target Information
The TLR8 protein is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. The TLR8 gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD antigen 288; TLR8; TLR8 isoform 1; unnamed protein product
Gene Aliases: CD288; hTLR8; IMD98; TLR-8
UniProt ID: (Human) Q9NR97
Entrez Gene ID: (Human) 51311, (Rat) 684440
regulation of protein phosphorylation
pattern recognition receptor signaling pathway
toll-like receptor signaling pathway
signal transduction
I-kappaB kinase/NF-kappaB signaling
response to virus
positive regulation of type I interferon production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interferon-gamma production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
toll-like receptor 8 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of innate immune response
defense response to virus
cellular response to mechanical stimulus
positive regulation of intracellular signal transduction
defense response
immune response
immunoglobulin mediated immune response
innate immune response
response to other organism
endolysosomal toll-like receptor signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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