Invitrogen

TSG101 Polyclonal Antibody

Name: TSG101 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-81094
Availability: InStock

高级验证

1个已发表图片

1 篇参考文献

查看其他26个TSG101抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

TSG101 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

TSG101 Antibody (PA5-81094) in ICC/IF

Immunofluorescence analysis of Tsg101 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TSG101 Polyclonal Antibody (Product # PA5-81094) at 1:250 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790), (1:20... View More

TSG101 Antibody in Immunoprecipitation (IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image TSG101 Polyclonal Antibody

产品信息

PA5-81094

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:500-1:50,000

免疫沉淀 (IP)

0.2-1 µL/mg of lysate

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to the N-terminus of the Human TSG101

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

100 µg

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2788341

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human TSG101.

靶标信息

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。