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Invitrogen
FTO Polyclonal Antibody
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FIGURE: 1 / 5
FTO Antibody (PA5-89791) in WB
Western Blot was performed using Anti-FTO Polyclonal Antibody (Product # PA5-89791) and a 58 and 58.2 kDa band corresponding to Alpha-ketoglutarate-dependent dioxygenase FTO was observed across cell lines and tissues tested . Nuclear enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), U-87 MG (Lane 2), Hep G2 (Lane 3), PC-3 (Lane 4), HeLa (Lane 5), MCF7 (Lane 6), Mouse Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-89791
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.40 mg/mL
Amount
40 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: 293T; Cellular Location: Nucleus, Nucleus speckle
Target Information
FTO is a novel protein and a member of the non-heme dioxygenase (Fe(II)-and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation patterns support this hypothesis and indicates that both 2-oxoglutarate and iron should be important for FTO function. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AlkB homolog 9; fat mass and obesity associated; fat mass and obesity-associated protein; fatso; intragenic FTO exon 9 containing transcript; m6A(m)-demethylase FTO; MGC5149; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenine demethylase; mRNA N(6)-methyladenosine demethylase FTO; protein fatso; protein fto; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; unnamed protein product
Gene Aliases: ALKBH9; BMIQ14; GDFD; IFEX9; mKIAA1752; RGD1305121
UniProt ID: (Human) Q9C0B1, (Mouse) Q8BGW1, (Rat) Q2A121
Entrez Gene ID: (Human) 79068, (Mouse) 26383, (Rat) 291905
protein binding
ferrous iron binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
oxidative RNA demethylase activity
oxidative DNA demethylase activity
RNA N6-methyladenosine dioxygenase activity
tRNA demethylase activity
molecular_function
osteoblast differentiation
temperature homeostasis
DNA dealkylation involved in DNA repair
regulation of lipid storage
snRNA processing
sensory perception of pain
positive regulation of insulin secretion involved in cellular response to glucose stimulus
regulation of multicellular organism growth
RNA repair
positive regulation of apoptotic process
regulation of respiratory system process
regulation of cytosolic calcium ion concentration
adipose tissue development
mRNA destabilization
regulation of white fat cell proliferation
regulation of brown fat cell differentiation
negative regulation of eating behavior
heart development
response to nutrient levels
response to bisphenol A
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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