Invitrogen

METTL4 Polyclonal Antibody

1 篇参考文献

查看其他7个METTL4抗体

问题与答案

问题与答案

问题与答案

Additional Information:

{{banner.description}}

{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

抗体检测数据 (6)

METTL4 Antibody in Immunocytochemistry (ICC/IF)

METTL4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

METTL4 Antibody (PA5-97202) in ICC/IF

Immunofluorescence analysis of METTL4 in NIH/3T3 cells. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

METTL4 Antibody in Immunocytochemistry (ICC/IF)

METTL4 Antibody in Immunocytochemistry (ICC/IF)

METTL4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

METTL4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

METTL4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

METTL4 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

METTL4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of METTL4 in NIH/3T3 cells. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

METTL4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of METTL4 in U2OS cells. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

METTL4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of METTL4 in paraffin-embedded human lung cancer. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

METTL4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of METTL4 in paraffin-embedded mouse kidney. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

METTL4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of METTL4 in paraffin-embedded rat testis. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

METTL4 Antibody in Western Blot (WB) — Western blot analysis of METTL4 in extracts of various cell lines. Samples were incubated with METTL4 Polyclonal antibody (Product # PA5-97202) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunogen image

METTL4 Polyclonal Antibody

Immunogen Image METTL4 Polyclonal Antibody

产品信息

PA5-97202

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein (or fragment)

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.91 mg/mL

291 µg

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunogen sequence: MSVVHQLSAG WLLDHLSFIN KINYQLHQHH EPCCRKKEFT TSVHFESLQM DSVSSSGVCA AFIASDSSTK PENDDGGNYE MFTRKFVFRP ELFDVTKPYI TPAVHKECQQ SNEKEDLMNG VKKEISISII GKKRKRCVVF NQGELDAMEY HTKIRELILD GSLQLIQEGL KSGFLYPLFE KQDKGSKPIT; Positive Samples: A-549, 293T, HeLa, MCF7, Mouse brain, Rat brain

靶标信息

METTL4 gene ontology annotations related to this gene include methyltransferase activity; nucleic acid binding.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Methyltransferase-like protein 4; N(6)-adenine-specific DNA methyltransferase METTL4; N(6)-adenine-specific methyltransferase METTL4; snRNA (2'-O-methyladenosine-N(6)-)-methyltransferase METTL4

查看更多收起

基因别名: 2410198H06Rik; A730091E08Rik; AV296509; HsT661; Mettl4; RGD1306451

查看更多收起

UniProt ID: (Mouse) Q3U034

查看更多收起

Entrez Gene ID: (Mouse) 76781, (Rat) 316731

查看更多收起

rRNA (adenine-N6,N6-)-dimethyltransferase activity nucleic acid binding protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity RNA metabolism protein RNA methyltransferase nucleic acid metabolism protein

nucleobase-containing compound metabolic process biological_process methylation