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          • Primary Antibodies ›
          • GSK3B Antibodies

          Invitrogen

          Phospho-GSK3B (Ser9) Polyclonal Antibody

          Advanced Verification
          View all (47) GSK3B antibodies

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          Cite Phospho-GSK3B (Ser9) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Advanced Verification (1)
          Phospho-GSK3B (Ser9) Antibody in Western Blot (WB)
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          Phospho-GSK3B (Ser9) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Phospho-GSK3B (Ser9) Antibody (PA5-97339) in WB

          Western Blot was performed using Anti-Phospho-GSK3B (Ser9) Polyclonal Antibody (Product # PA5-97339) and a 50 kDa band corresponding to Glycogen synthase kinase-3 beta was observed to be induced upon Calyculin A treatment in HeLa cells, and upregulated upon EGF treatment and downregulated upon SB216763 followed by EGF treatment in A549 cells. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-GSK3B (Ser9) Antibody in Western Blot (WB)
          Phospho-GSK3B (Ser9) Antibody in Western Blot (WB)
          Phospho-GSK3B (Ser9) Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-GSK3B (Ser9) Polyclonal Antibody

          Product Details

          PA5-97339

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:100-1:500
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.77 mg/mL

          Amount

          77 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2809141

          Product Specific Information

          Positive Samples: HeLa; Cellular Location: Cell membrane, Cytoplasm, Nucleus

          Immunogen sequence: TTSFAE

          Target Information

          GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: EC 2.7.11.26; FA; Factor A; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; GSK3B; kinase; pp37; Serine/threonine-protein kinase GSK3B; unnamed protein product

          View more View less

          Gene Aliases: 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; GSK3B

          View more View less

          UniProt ID: (Mouse) Q9WV60, (Rat) P18266

          View more View less

          Entrez Gene ID: (Human) 2932, (Mouse) 56637, (Rat) 84027

          View more View less

          Function(s)
          protease binding p53 binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding protein kinase A catalytic subunit binding dynactin binding tau protein binding tau-protein kinase activity NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding dynein complex binding scaffold protein binding protein serine kinase activity nucleotide binding RNA polymerase II transcription factor binding integrin binding transcription factor binding transferase activity transferase activity, transferring phosphorus-containing groups ionotropic glutamate receptor binding non-receptor serine/threonine protein kinase
          Process(es)
          epithelial to mesenchymal transition positive regulation of cell-matrix adhesion heart valve development glycogen metabolic process protein phosphorylation ER overload response mitochondrion organization meiosis I circadian rhythm insulin receptor signaling pathway negative regulation of signal transduction positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition positive regulation of protein processing regulation of neuron projection development Wnt signaling pathway peptidyl-serine phosphorylation viral protein processing hippocampus development establishment of cell polarity maintenance of cell polarity cell differentiation negative regulation of cell migration regulation of axon extension neuron projection development negative regulation of protein complex assembly positive regulation of protein complex assembly positive regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway negative regulation of TOR signaling positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of type I interferon production regulation of protein localization regulation of microtubule-based process response to endoplasmic reticulum stress intracellular signal transduction cellular response to interleukin-3 regulation of circadian rhythm regulation of apoptotic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process protein kinase B signaling positive regulation of neuron apoptotic process positive regulation of cell differentiation negative regulation of osteoblast differentiation negative regulation of glycogen biosynthetic process positive regulation of cilium assembly positive regulation of protein catabolic process negative regulation of smoothened signaling pathway protein autophosphorylation regulation of protein export from nucleus positive regulation of protein export from nucleus regulation of dendrite morphogenesis regulation of axonogenesis canonical Wnt signaling pathway excitatory postsynaptic potential intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress regulation of microtubule cytoskeleton organization negative regulation of calcineurin-NFAT signaling cascade superior temporal gyrus development cellular response to retinoic acid cellular response to glucocorticoid stimulus negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand presynaptic modulation of chemical synaptic transmission neuron projection organization regulation of microtubule anchoring at centrosome beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of substrate adhesion-dependent cell spreading regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of long-term synaptic potentiation positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein localization to cilium negative regulation of TORC2 signaling negative regulation of dopaminergic neuron differentiation cellular response to beta-amyloid negative regulation of protein localization to centrosome positive regulation of protein localization to centrosome positive regulation of establishment of protein localization negative regulation of type B pancreatic cell development negative regulation of glycogen (starch) synthase activity positive regulation of cardiac muscle cell differentiation negative regulation of mesenchymal stem cell differentiation re-entry into mitotic cell cycle carbohydrate metabolic process regulation of gene expression by genetic imprinting protein export from nucleus cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development nervous system development axonogenesis myoblast fusion cell proliferation organ morphogenesis negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation phosphorylation cell migration negative regulation of protein binding positive regulation of peptidyl-serine phosphorylation protein localization to microtubule positive regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter regulation of neuronal synaptic plasticity rhythmic process negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus cellular response to mechanical stimulus positive regulation of stem cell differentiation response to lithium ion response to drug
          It has to be done as per old AB suggested Products section.

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