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Invitrogen

Phospho-ERK1 (Tyr204) Polyclonal Antibody

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Phospho-ERK1 (Tyr204) Polyclonal Antibody

Product Details

PA5-97359

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
-

Immunocytochemistry (ICC/IF)

1:100-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around Y204 of human ERK1 (NP_0027372)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.84 mg/mL

Amount

184 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2809161

Product Specific Information

Positive Samples: Jurkat; Cellular Location: Cytoplasm, Nucleus

Immunogen sequence: TEYVA

Target Information

MAPK3 (ERK1) is a serine/threonine kinase that is an important activator of p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Functionally, ERK1 is involved with a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. ERK1 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ERK; ERK-1; Erk1; cancer susceptibility protein; ERK1b; ERK1c; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAP kinase2; MAPK 1; MAPK 3; MAPK2; Microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MNK1; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; unnamed protein product

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Gene Aliases: ERK-1; ERK1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MAPK3; MNK1; Mtap2k; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3

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UniProt ID: (Rat) P21708, (Mouse) Q63844

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Entrez Gene ID: (Human) 5595, (Rat) 50689, (Mouse) 26417

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Function(s)
phosphotyrosine binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding phosphatase binding identical protein binding protein serine kinase activity DNA-binding transcription factor binding scaffold protein binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade negative regulation of T cell mediated immune response to tumor cell protein phosphorylation cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway insulin receptor signaling pathway positive regulation of macrophage chemotaxis Schwann cell development phosphorylation sensory perception of pain BMP signaling pathway positive regulation of cyclase activity positive regulation of telomere maintenance regulation of stress-activated MAPK cascade cellular response to amino acid starvation intracellular signal transduction peptidyl-tyrosine autophosphorylation ERBB2-ERBB3 signaling pathway myelination signal transduction in response to DNA damage positive regulation of transcription from RNA polymerase II promoter insulin-like growth factor receptor signaling pathway modulation of synaptic transmission stress-activated MAPK cascade regulation of cytoskeleton organization interleukin-34-mediated signaling pathway ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus caveolin-mediated endocytosis regulation of Golgi inheritance negative regulation of cholesterol efflux positive regulation of macrophage proliferation positive regulation of neuroinflammatory response negative regulation of TORC1 signaling regulation of early endosome to late endosome transport MAPK import into nucleus positive regulation of protein phosphorylation transcription, DNA-templated protein complex assembly apoptotic process cellular response to DNA damage stimulus DNA damage induced protein phosphorylation response to toxic substance organ morphogenesis peptidyl-serine phosphorylation arachidonic acid metabolic process lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide positive regulation of histone phosphorylation positive regulation of histone acetylation response to exogenous dsRNA positive regulation of translation positive regulation of transcription, DNA-templated regulation of sequence-specific DNA binding transcription factor activity cartilage development lung morphogenesis trachea formation negative regulation of apolipoprotein binding cell cycle signal transduction
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