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Invitrogen
PCAF Polyclonal Antibody
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图: 1 / 6
PCAF Antibody (PA5-99179) in ICC/IF
Immunocytochemistry analysis of PCAF in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PCAF polyclonal antibody (Product # PA5-99179) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-99179
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human KAT2B(Accession Q92831), corresponding to amino acid residues K800-K832.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total PCAF.
靶标信息
PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles. PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. PCAF also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and acetylation regulating nucleocytoplasmic shutting of TBX5.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Lysine acetyltransferase 2B; P/ PCAF; P/CAF; P300/CBP-associated factor; Spermidine acetyltransferase KAT2B
基因别名: A930006P13Rik; AI461839; AW536563; KAT2B; p/CAF; PCAF
UniProt ID: (Mouse) Q9JHD1
Entrez Gene ID: (Rat) 301164, (Mouse) 18519
RNA polymerase II regulatory region sequence-specific DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
histone acetyltransferase activity
lysine N-acetyltransferase activity, acting on acetyl phosphate as donor
cyclin-dependent protein serine/threonine kinase inhibitor activity
protein binding
transcription factor binding
acetyltransferase activity
protein kinase binding
protein complex binding
histone acetyltransferase binding
histone deacetylase binding
N-acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
chromatin remodeling
protein acetylation
memory
negative regulation of cell proliferation
regulation of protein ADP-ribosylation
positive regulation of neuron projection development
histone acetylation
N-terminal peptidyl-lysine acetylation
internal peptidyl-lysine acetylation
peptidyl-lysine acetylation
cellular response to insulin stimulus
cellular response to oxidative stress
positive regulation of chromatin binding
positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter
histone H3 acetylation
histone H3-K9 acetylation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of transcription, DNA-templated
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
cellular response to parathyroid hormone stimulus
positive regulation of histone H3-K14 acetylation
positive regulation of histone H3-K9 acetylation
transcription, DNA-templated
regulation of transcription, DNA-templated
cell cycle
rhythmic process
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