Transgenic mice expressing a cameleon fluorescent Ca2+ indicator in astrocytes and Schwann cells allow study of glial cell Ca2+ signals in situ and in vivo.
Authors:Atkin SD, Patel S, Kocharyan A, Holtzclaw LA, Weerth SH, Schram V, Pickel J, Russell JT,
Journal:J Neurosci Methods
PubMed ID:19454294
'Glial cell Ca2+ signals play a key role in glial-neuronal and glial-glial network communication. Numerous studies have thus far utilized cell-permeant and injected Ca2+ indicator dyes to investigate glial Ca2+ signals in vitro and in situ. Genetically encoded fluorescent Ca2+ indicators have emerged as novel probes for investigating cellular Ca2+ ... More
Optimizing a waveguide-based sandwich immunoassay for tumor biomarkers: evaluating fluorescent labels and functional surfaces.
Authors:Mukundan H, Xie H, Anderson AS, Grace WK, Shively JE, Swanson BI,
Journal:Bioconjug Chem
PubMed ID:19173652
'The sensor team at the Los Alamos National Laboratory has developed a waveguide-based optical biosensor for the detection of biomarkers associated with disease. We have previously demonstrated the application of this technology to the sensitive detection of carcinoembryonic antigen in serum and nipple aspirate fluid from breast cancer patients. In ... More
Targeted cellular delivery of quantum dots loaded on and in biotinylated liposomes.
Authors:Sigot V, Arndt-Jovin DJ, Jovin TM,
Journal:Bioconjug Chem
PubMed ID:20715851
'We describe the preparation, biophysical characterization, and receptor-mediated cellular internalization of biotinylated lipid particles (BLPs) loaded on the surface and internally with two distinct (colors) of quantum dot (QD) probes. BLPs were formulated with 1.4 and 2.7 mol % PEG-lipids containing either a fusogenic or pH-sensitive lipid to promote bilayer ... More
Quantum dot photon statistics measured by three-dimensional particle tracking.
Authors:McHale K, Berglund AJ, Mabuchi H
Journal:Nano Lett
PubMed ID:17949048
'We present an instrument for performing correlation spectroscopy on single fluorescent particles while tracking their Brownian motion in three dimensions using real-time feedback. By tracking CdSe/ZnS quantum dots in water (diffusion coefficient approximately 20 mum2/s), we make the first measurements of photon antibunching (at approximately 10 ns) on single fluorophores ... More
Bioconjugation of the estrogen receptor hER(a) to a quantum dot dye for a controlled immobilization on a SiO(2) surface.
Authors:Cherkouk C, Rebohle L, Skorupa W,
Journal:J Colloid Interface Sci
PubMed ID:21216405
'We investigated the immobilization of the estrogen receptor hER(a) on silanized SiO(2) surfaces for biosensor applications. The conjugation of the estrogen receptor hER(a) to the quantum dot dye QD655 was achieved. In order to obtain an optimal immobilization of the estrogen receptor hER(a) on the functionalized SiO(2) surface, the bioconjugate ... More