'Our understanding of the biologic effects (including toxicity) of nanomaterials is incomplete. In vivo animal studies remain the gold standard; however, widespread testing remains impractical, and the development of in vitro assays that correlate with in vivo activity has proven challenging. Here, we demonstrate the feasibility of analyzing in vitro ... More
Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
AuthorsLiu DS, Phipps WS, Loh KH, Howarth M, Ting AY,
JournalACS Nano
PubMed ID23181687
'We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second ... More
Dynamic visualization of RGD-quantum dot binding to tumor neovasculature and extravasation in multiple living mouse models using intravital microscopy.
AuthorsSmith BR, Cheng Z, De A, Rosenberg J, Gambhir SS,
JournalSmall
PubMed ID20862677
In this work, we exploited intravital microscopy to develop in vivo nanoparticle binding and extravasation assays, comparing the experimental RGD-Qdot condition with controls in various tumor models in living mice. ... More
Sentinel lymph node imaging using quantum dots in mouse tumor models.
We demonstrate that quantum dots injected into two model tumors rapidly migrate to sentinel lymph nodes. PEG-coated quantum dots having terminal carboxyl, amino, or methoxyl groups all migrated from the tumor to surrounding lymph nodes similarly. Passage from the tumor through lymphatics to adjacent nodes could be visualized dynamically through ... More
microPET-based biodistribution of quantum dots in living mice.
This study evaluates the quantitative biodistribution of commercially available CdSe quantum dots (QD) in mice. (64)Cu-Labeled 800- or 525-nm emission wavelength QD (21- or 12-nm diameter), with or without 2,000 MW (molecular weight) polyethylene glycol (PEG), were injected intravenously into mice (5.55 MBq/25 pmol QD) and studied using well counting ... More
Imaging epidermal growth factor receptor expression in vivo: pharmacokinetic and biodistribution characterization of a bioconjugated quantum dot nanoprobe.
AuthorsDiagaradjane P, Orenstein-Cardona JM, E Colón-Casasnovas N, Deorukhkar A, Shentu S, Kuno N, Schwartz DL, Gelovani JG, Krishnan S,
JournalClin Cancer Res
PubMed ID18245533
PURPOSE: To develop and validate an optical imaging nanoprobe for the discrimination of epidermal growth factor (EGF) receptor (EGFR)-overexpressing tumors from surrounding normal tissues that also expresses EGFR. EXPERIMENTAL DESIGN: Near-infrared (NIR) quantum dots (QD) were coupled to EGF using thiol-maleimide conjugation to create EGF-QD nanoprobes. In vitro binding affinity ... More