NucBlue™ Fixed Cell ReadyProbes™ 试剂 (DAPI)
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NucBlue™ Fixed Cell ReadyProbes™ 试剂 (DAPI)
Invitrogen™

NucBlue™ Fixed Cell ReadyProbes™ 试剂 (DAPI)

DAPI 是一种常用的固定细胞细胞核复染剂,与 DNA 结合后会发出蓝色荧光。借助于 NucBlue™ Fixed Cell ReadyProbes™ 试剂,我们配制了这种经典染色剂高纯度形式的室温稳定溶液,用方便使用的滴瓶包装了解更多信息
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货号数量
R376066 管
货号 R37606
价格(CNY)
1,262.00
飞享价
Ends: 31-Dec-2025
1,699.00
共减 437.00 (26%)
Each
添加至购物车
数量:
6 管
价格(CNY)
1,262.00
飞享价
Ends: 31-Dec-2025
1,699.00
共减 437.00 (26%)
Each
添加至购物车
DAPI 是一种常用的固定细胞细胞核复染剂,与 DNA 结合后会发出蓝色荧光。借助于 NucBlue™ Fixed Cell ReadyProbes™ 试剂,我们配制了这种经典染色剂高纯度形式的室温稳定溶液,用方便使用的滴瓶包装。每毫升只需滴注两滴,即可对细胞进行染色。

还可以: 12.5X 浓度 (5 µM) 的 DAPI(4',6-二脒基-2-苯基吲哚二盐酸盐)水溶液。

•无需稀释、称重或移液
• 滴瓶方便取用—每毫升只需滴加2滴
• 室温稳定—可放置在视野范围内或细胞培养区域,取用方便• DAPI 可被紫外光激发,可通过蓝色/青色滤光片进行检测

查看其他 ReadyProbes™ 细胞染色试剂
查看其他成像用细胞核染色剂

细胞成像应用
DAPI 是一种经典的荧光染料,广泛用于固定细胞的细胞核染色。在荧光显微镜下,DAPI 被紫外光激发,可通过蓝色/青色滤光片进行检测(图1)。

使用建议
在大多数情况下,每毫升滴加2滴 NucBlue™ 固定细胞染色剂、孵育15至30分钟即可产生明亮的细胞核染色;但是,对于某些细胞类型、条件和应用,可能需要优化。在这种情况下,只需增加或减少滴数,直至获得最佳染色强度即可。
•与 DNA 结合后,NucBlue™ 固定细胞染色剂可被 360 nm 的紫外光激发,最大发射波长为 460 nm。可通过蓝/青色滤光片进行检测,如 DAPI 滤光片、蓝色 GFP 滤光片或 Semrock BrightLine™ Alexa Fluor™ 350 染料滤光片套件。
•作为固定细胞成像实验的首选蓝色细胞核染色剂,NucBlue™ 固定细胞染色剂非常适用于抗体应用。
仅供科研使用。不可用于诊断程序。
规格
颜色蓝色
检测方法荧光
染料类型DAPI
发射可见光
激发波长范围360⁄460
适用于(设备)荧光显微镜、流式细胞仪
形式液体
产品线Molecular Probes™
数量6 管
运输条件室温
标签类型Fluorescent Dye
产品类型核酸染色剂
亚细胞定位细胞核/核仁
Unit SizeEach
内容与储存
6 × 2.5 mL 滴瓶

在 ≤25°C 下储存

常见问题解答 (FAQ)

My DAPI labeled samples have a strong blue background signal immediately after mounting. What can I do to fix this?

Some mounting medias can have strong blue autofluorescence. If you are seeing a high blue background, it could be coming from the mountant. Try labeling the sample and view it before (using a wet mount in buffer) and after mounting to determine if the background signal is coming from the mounting media or the sample itself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer an alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538)?

We do not have a direct alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538). However, we do have alternative primary and secondary antibodies, as well as reagents, that can be used for trilineage differentiation. Please note that we have not internally validated the use of all these reagents together.

We recommend the following primary antibodies:

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)) (Cat. No. MA5-11547)

alpha-Fetoprotein Monoclonal Antibody (AFP3) (Cat. No. 14-6583-80)

beta-3 Tubulin Monoclonal Antibody (2G10) (Cat. No. MA1-118)

We recommend the following secondary antibodies:

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 555 (Cat. No. A21137)

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 594 (Cat. No. A21135)

https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG1-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21121

We recommend using the following reagents:

https://www.thermofisher.com/order/catalog/product/88-8824-00

NucBlue Fixed Cell ReadyProbes Reagent (DAPI) (Cat. No. R37606)

Blocker BSA (Cat. No. 37520)

DPBS (10X), no calcium, no magnesium (Cat. No. 14200075)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.