Limb bud mesenchyme permits seminiferous cord formation in the mouse fetal testis but subsequent testosterone output is markedly affected by the sex of the donor stromal tissue.
AuthorsMoreno-Mendoza N, Herrera-Muñoz J, Merchant-Larios H
JournalDev Biol
PubMed ID7750656
Mesonephric stromal cells have previously been shown to migrate into the genital ridge and to be necessary for seminiferous cord formation in organ culture. Here, we asked whether there is a specific requirement for mesonephric stromal cells or whether another source of mesonephric stromal cells can be substituted. Hindlimb buds ... More
Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.
AuthorsVan Amersfoort ES, Van Strijp JA
JournalCytometry
PubMed ID7875036
A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and ... More
The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes.
AuthorsAndrade W, Seabrook TJ, Johnston MG, Hay JB
JournalJ Immunol Methods
PubMed ID8765171
'In this study we examined the new cell dye CM-DiI for tracking the migration of lymphocytes from blood to lymph. This lipophilic marker intercalates in the plasma membrane like the PKH dyes and older DiI derivatives. The stability and intensity of staining achieved with these dyes is better than most ... More
Mechanisms of adoptive immunotherapy: improved methods for in vivo tracking of tumor-infiltrating lymphocytes and lymphokine-activated killer cells.
AuthorsWallace PK, Palmer LD, Perry-Lalley D, Bolton ES, Alexander RB, Horan PK, Yang JC, Muirhead KA
JournalCancer Res
PubMed ID8485722
'Adoptive immunotherapy with tumor-infiltrating lymphocytes (TIL) and lymphokine-activated killer cells has been demonstrated to mediate regression of tumors in murine models and in selected patients with advanced cancer. Improved methods for monitoring immune cell traffic, particularly to sites of tumor, are needed to elucidate mechanisms of antitumor activity and optimize ... More
A flow cytometric method to assess antigen-specific proliferative responses of different subpopulations of fresh and cryopreserved human peripheral blood mononuclear cells.
AuthorsAllsopp CE, Nicholls SJ, Langhorne J
JournalJ Immunol Methods
PubMed ID9692869
'We have used PKH26 dye, which is incorporated stably into the membrane of cells, to determine, using flow cytometry, lymphocyte proliferative responses to the antigen tetanus toxoid in fresh and cryopreserved samples. Measuring cell proliferation with this dye has advantages over either 3H-thymidine or Bromodeoxyuridine (BrdU). Whereas the existing methods ... More
Direct contact with stroma inhibits proliferation of human long-term culture initiating cells.
AuthorsVerfaillie CM, Catanzaro P
JournalLeukemia
PubMed ID8642869
'Significantly less long-term culture initiating cells (LTC-IC) are recovered from cultures in which progenitors are cultured in contact with stroma (stroma-contact) than when cultured separated from stroma by a transwell (stroma-noncontact). This suggests that direct contact with stroma inhibits either proliferation or survival of LTC-IC. Using the membrane intercallating fluorochrome, ... More
Migration of Langerhans cells in an in vitro organ culture system: IL-6 and TNF-alpha are partially responsible for migration into the epidermis.
AuthorsSaitoh A, Yasaka N, Osada A, Nakamura K, Furue M, Tamaki K
JournalJ Dermatol Sci
PubMed ID10215188
'Although it is well established that epidermal Langerhans cells (LC) originate from bone marrow, little is known about the mechanism of this migration into the epidermis from bone marrow. In order to clarify the mechanism of this migration, we constructed an in vitro model. LC were depleted by daily topical ... More
Effects of supravital fluorochromes used to analyze the in vivo homing of murine lymphocytes on cellular function.
AuthorsSamlowski WE, Robertson BA, Draper BK, Prystas E, McGregor JR
JournalJ Immunol Methods
PubMed ID1960398
'A number of supravital fluorochromes are available to study lymphocyte homing in vivo. These include fluorescein isothiocyanate (FITC), which binds to cell surface proteins; Hoechst 33342, which binds to AT rich regions of cellular DNA; and the lipid bilayer incorporated dyes PKH-2 and PKH-26. The relative advantages and disadvantages of ... More
Molecular mechanisms of erythrophagocytosis. Characterization of the senescent erythrocytes that are phagocytized by macrophages.
'We have recently developed a flow cytometric assay for the quantitation of erythrophagocytosis, using PKH 26-labeled erythrocytes as the target cells. Using this assay we have shown that there is extensive phagocytosis of desialylated erythrocytes. Furthermore, we have demonstrated that it is the densest population of erythrocytes obtained on a ... More
Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole.
AuthorsHenriques C, de Souza W
JournalParasitol Res
PubMed ID10726992
'Leishmania amazonensis presents two developmental stages that gain access to the host macrophage through phagocytosis. The protozoan resides in a membrane-bound compartment, the parasitophorous vacuole (PV), which can fuse with the endocytic system. For evaluation of the parasite/host-cell interaction process and of PV biogenesis, the two parasite forms or host-cell ... More
Usefulness of PKHs for studying cell proliferation.
AuthorsBoutonnat J, Barbier M, Rousselle C, Muirhead KA, Mousseau M, Seigneurin D, Ronot X
JournalC R Acad Sci III
PubMed ID9879469
'Classical methods for proliferative assessment (such as tritiated thymidine or bromodeoxyuridine (BrdUrd) incorporation) need sample fixation. As an alternative, we have evaluated the use of a dye dilution method using PKH26 to determine the rate and extent of proliferation in cell lines. Flow cytometric analysis associated with modelling software makes ... More
Hematopoietic stem cell tracking in vivo: a comparison of short-term and long-term repopulating cells.
AuthorsLanzkron SM, Collector MI, Sharkis SJ
JournalBlood
PubMed ID10068664
'We have previously demonstrated that we could separate long-term repopulating stem cells from cells that provided radioprotection (short-term repopulating cells) on the basis of size and suggested that this might be due to the quiescent nature of long-term repopulating cells. To further define the activity of these populations, we used ... More
The properties of hNT cells following transplantation into the subventricular zone of the neonatal forebrain.
AuthorsZigova T, Pencea V, Sanberg PR, Luskin MB
JournalExp Neurol
PubMed ID10785441
'Neurons derived from the human teratocarcinoma cell line (hNT) establish structural polarity and a fully mature phenotype following transplantation into the rodent brain. Here we describe the transplantation of hNT cells into the anterior part of neonatal subventricular zone (SVZa), which is a prolific region of neuronal progenitor cells. Ordinarily, ... More
Murine stromal cell line HESS-5 maintains reconstituting ability of Ex vivo-generated hematopoietic stem cells from human bone marrow and cytokine-mobilized peripheral blood.
AuthorsShimakura Y, Kawada H, Ando K, Sato T, Nakamura Y, Tsuji T, Kato S, Hotta T
JournalStem Cells
PubMed ID10840071
'Human bone marrow (BM) or mobilized peripheral blood (mPB) CD34(+) cells have been shown to loose their stem cell quality during culture period more easily than those from cord blood (CB). We previously reported that human umbilical CB stem cells could effectively be expanded in the presence of human recombinant ... More
Maintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors.
AuthorsLansdorp PM, Dragowska W
JournalExp Hematol
PubMed ID7689481
'We previously described that cells with a CD34+CD71lo phenotype from adult human bone marrow are maintained at constant numbers in long-term suspension cultures supplemented with interleukin-6 (IL-6), IL-3, mast growth factor (MGF) (a c-kit ligand), and erythropoietin (Epo). In view of the large increase in cell numbers in such cultures ... More
Organ-selective homing defines engraftment kinetics of murine hematopoietic stem cells and is compromised by Ex vivo expansion.
AuthorsSzilvassy SJ, Bass MJ, Van Zant G, Grimes B
JournalBlood
PubMed ID10029584
'Hematopoietic reconstitution of ablated recipients requires that intravenously (IV) transplanted stem and progenitor cells "home" to organs that support their proliferation and differentiation. To examine the possible relationship between homing properties and subsequent engraftment potential, murine bone marrow (BM) cells were labeled with fluorescent PKH26 dye and injected into lethally ... More
Flow cytometric analysis of intercellular adhesion between B-cell precursor acute lymphoblastic leukemic cells and bone marrow stromal cells.
AuthorsAshley DM, Bol SJ, Tucker DP, Waugh CM, Kannourakis G
JournalLeukemia
PubMed ID7845030
'The growth of B-cell precursor acute lymphoblastic leukemic (BCP ALL) cells in vitro is dependent on interactions with bone marrow (BM) stromal cells. We have recently demonstrated that the rate of cell division of BCP ALL cells increases when cultured in direct contact with BM stromal cells. In this study ... More
Human bone marrow stromal cell contact and soluble factors have different effects on the survival and proliferation of paediatric B-lineage acute lymphoblastic leukaemic blasts.
AuthorsAshley DM, Bol SJ, Kannourakis G
JournalLeuk Res
PubMed ID8182924
'Recent studies have confirmed that in vitro viability and proliferation of precursor B-cell leukaemia (ALL) cells are linked to the presence of bone marrow derived stromal cells. To investigate whether this effect is mediated by direct contact or through the action of soluble factors, using a method we have recently ... More
Drug delivery into the arterial wall: a time-course study with use of a lipophilic dye.
AuthorsConsigny PM, Miller KT
JournalJ Vasc Interv Radiol
PubMed ID8000122
'PURPOSE: One potential approach to the prevention of restenosis after angioplasty is to deliver antiproliferative agents directly to the angioplasty site. The purpose of this study was to determine the time course of drug penetration into the media of the balloon-dilated artery. MATERIALS AND METHODS: Balloon angioplasty of the left ... More
Random walk of processive, quantum dot-labeled myosin Va molecules within the actin cortex of COS-7 cells.
AuthorsNelson SR, Ali MY, Trybus KM, Warshaw DM,
JournalBiophys J
PubMed ID19619465
'Myosin Va (myoVa) is an actin-based intracellular cargo transporter. In vitro experiments have established that a single myoVa moves processively along actin tracks, but less is known about how this motor operates within cells. Here we track the movement of a quantum dot (Qdot)-labeled myoVa HMM in COS-7 cells using ... More
Accumulation of macrophages with dendritic cell characteristics in the pulmonary response to Listeria.
'Pulmonary immunity reflects a balance between proinflammatory and immunosuppressive factors in the lung. To determine the immune activities of exudate macrophages in the pulmonary immune response, Lewis rats were injected intratracheally with heat-killed Listeria (HKL), labeled ex vivo with the lipophilic dye PKH-26. At 24 h, macrophages from bronchoalveolar lavage ... More
Regionalized expression of myosin isoforms in heterotypic myotubes formed from embryonic and fetal rat myoblasts in vitro.
AuthorsPin CL, Merrifield PA
JournalDev Dyn
PubMed ID9056645
'The development of mammalian limb muscles involves the appearance and fusion of at least two separate populations of muscle precursor cells. These two populations, termed embryonic and fetal myoblasts, are first detected within the limb bud at different stages of development. We have previously demonstrated that, in the rat, each ... More
Mast cells migrate from blood to brain.
AuthorsSilverman AJ, Sutherland AK, Wilhelm M, Silver R
JournalJ Neurosci
PubMed ID10627616
'It is well established that mast cells (MCs) occur within the CNS of many species. Furthermore, their numbers can increase rapidly in adults in response to altered physiological conditions. In this study we found that early postpartum rats had significantly more mast cells in the thalamus than virgin controls. Evidence ... More
Viable bone marrow stromal cells are required for the in vitro survival of B-cell precursor acute lymphoblastic leukemic cells.
AuthorsAshley DM, Bol SJ, Kannourakis G
JournalLeuk Res
PubMed ID7869739
'The growth of B-cell precursor acute lymphoblastic leukemic (BCP ALL) cells in vitro is dependent on interactions with bone marrow (BM) stromal cells. We have recently demonstrated that the rate of cell division of BCP ALL cells increases when cultured in direct contact with BM stromal cells. A number of ... More
Symmetry of initial cell divisions among primitive hematopoietic progenitors is independent of ontogenic age and regulatory molecules.
AuthorsHuang S, Law P, Francis K, Palsson BO, Ho AD
JournalBlood
PubMed ID10515863
'We have developed a time-lapse camera system to follow the replication history and the fate of hematopoietic stem cells (HSC) at a single-cell level. Combined with single-cell culture, we correlated the early replication behavior with colony development after 14 days. The membrane dye PKH26 was used to monitor cell division. ... More
A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens.
AuthorsGivan AL, Fisher JL, Waugh M, Ernstoff MS, Wallace PK
JournalJ Immunol Methods
PubMed ID10594357
'Fluorescent dyes that stain cell membranes or cytoplasm and then partition between daughter cells at division have been used in conjunction with flow cytometry to measure the proliferation of cells. In this paper, using peripheral blood mononuclear cells responding to tetanus toxoid, we describe an extension of this dye methodology ... More
A study on the engraftment and trafficking of bovine peripheral blood leukocytes in severe combined immunodeficient mice.
AuthorsGreenwood JD, Croy BA
JournalVet Immunol Immunopathol
PubMed ID8256436
'Bovine lymphoid cells were engrafted into untreated and into sublethally irradiated scid/scid mice, following intraperitoneal (i.p.) injection of bovine peripheral blood leukocytes (PBL). To distinguish bovine from murine cells, bovine PBL were stained prior to i.p. injection with a fluorescent cell linker compound, PKH26-GL and, after cell recovery, labelled with ... More
Media that support the growth and differentiation of oligodendrocytes do not induce olfactory ensheathing cells to express a myelinating phenotype.
AuthorsDoucette R, Devon R
JournalGlia
PubMed ID8056440
'The glial cells that ensheath olfactory axons are referred to as ensheathing cells. In vivo, these non-myelinating glial cells express a mixture of astrocyte-specific and Schwann cell-specific phenotypic features with the former cellular phenotype predominating, but in vitro can assemble a myelin sheath when co-cultured with dorsal root ganglion neurons. ... More
Flt3 ligand can promote survival and macrophage development without proliferation in myeloid progenitor cells.
AuthorsNicholls SE, Winter S, Mottram R, Miyan JA, Whetton AD
JournalExp Hematol
PubMed ID10210324
'Flt3 ligand elicits a variety of effects on early hemopoietic progenitors by occupying its cognate receptor, Flt3, a member of the type III tyrosine kinase receptor family. The cytokines macrophage colony-stimulating factor (M-CSF) and stem cell factor (SCF) bind to related members of this tyrosine kinase receptors family, c-fms and ... More
Phototoxicity of the fluorescent membrane dyes PKH2 and PKH26 on the human hematopoietic KG1a progenitor cell line.
AuthorsOh DJ, Lee GM, Francis K, Palsson BO
JournalCytometry
PubMed ID10404146
'BACKGROUND: The phototoxic effects of the well-known fluorescent membrane dyes PKH2 and PKH26 have been unknown, although their use in cell tracking experiments has increased dramatically. To eliminate the phototoxicity-induced alteration in cell function and morphology, it is essential to examine the suspicious phototoxicity of these dyes. METHODS: Chemical and ... More
Thrombopoietin, kit ligand, and flk2/flt3 ligand together induce increased numbers of primitive hematopoietic progenitors from human CD34+Thy-1+Lin- cells with preserved ability to engraft SCID-hu bone.
AuthorsLuens KM, Travis MA, Chen BP, Hill BL, Scollay R, Murray LJ
JournalBlood
PubMed ID9454750
'CD34(+)Thy-1(+)Lin- cells are enriched for primitive hematopoietic progenitor cells (PHP), as defined by the cobblestone area-forming cell (CAFC) assay, and for bone marrow (BM) repopulating hematopoietic stem cells (HSC), as defined by the in vivo SCID-hu bone assay. We evaluated the effects of different cytokine combinations on BM-derived PKH26-labeled CD34(+)Thy-1(+)Lin- ... More
In vitro replication dynamics of human culture-derived macrophages in a long term serum-free system.
AuthorsBennett S, Por SB, Cooley MA, Breit SN
JournalJ Immunol
PubMed ID7680690
'Human peripheral blood monocytes maintained in a long term serum-free system were found to undergo extensive replication. Newly replicated culture-derived macrophages initially appeared as colonies of small cells on the adherent monolayer. After the appearance of these colonies, large numbers of nonadherent macrophages were observed. Using PKH26, a fluorescent tracking ... More
The surface coat of infective larvae of Trichinella spiralis.
AuthorsModha J, Roberts MC, Robertson WM, Sweetman G, Powell KA, Kennedy MW, Kusel JR
JournalParasitology
PubMed ID10363284
'The surface coat of the infective larvae of the parasitic nematode Trichinella spiralis was characterized with respect to its biophysical properties, morphology and composition. Labelling of larvae with the fluorescent surface probe PKH26 was lost after activation (by incubation in mammalian medium containing trypsin and bile), or following pronase treatment. ... More
Sorting out of limb bud cells in monolayer culture.
AuthorsWada N, Ide H
JournalInt J Dev Biol
PubMed ID7981044
'To examine differences in the surface properties of limb bud cells, we mixed cells from the progress zone at different stages or from different positions along proximodistal axis of chick and quail wing limb buds. To identify the origin of cells, a chick-specific antibody was used in a mixed culture ... More
Identification of distinct luminal domains for macromolecules, erythrocytes, and leukocytes within mammalian capillaries.
AuthorsVink H, Duling BR
JournalCirc Res
PubMed ID8781491
'A thick endothelial surface coat consisting of the glycocalyx and associated plasma proteins has been hypothesized to reduce functional capillary volume available for flowing plasma macromolecules and blood cells. The purpose of this study was to compare anatomic and functional capillary diameters available for macromolecules, RBCs, and WBCs in hamster ... More
Retention of quiescent hematopoietic cells with high proliferative potential during ex vivo stem cell culture.
AuthorsYoung JC, Varma A, DiGiusto D, Backer MP
JournalBlood
PubMed ID8555476
'Human CD34+/Thy-1+/Lin- hematopoietic cells purified from bone marrow (BM) or mobilized peripheral blood (MPB) are highly enriched for pluripotent stem cells. Ex vivo expansion of this population is proposed as a means of providing accelerated short-term, as well as long-term, engraftment after myeloablative therapy. Here we demonstrate that primitive quiescent ... More
A versatile flow cytometry-based assay for the determination of short- and long-term natural killer cell activity.
AuthorsJohann S, Blümel G, Lipp M, Förster R
JournalJ Immunol Methods
PubMed ID7561131
'A flow cytometry based method has been developed to assess natural killer (NK) cell activity in both short-term (4 h) and long-term (18 h) NK assays. Target cells were either labeled with PKH-2, c''FDA or D275. Simultaneously, dead cells were identified by counter-staining with the nuclear dye propidium iodide. Using ... More
Extension of osmolality-induced podia is observed from fluorescently labeled hematopoietic cell lines in hyperosmotic medium.
AuthorsOh DJ, Martinez AR, Lee GM, Francis K, Palsson BO
JournalCytometry
PubMed ID10805930
'BACKGROUND: Since the description of long podia extended by hematopoietic cells and cell lines, the reliable elicitation of podia extensions is needed to study these podia systemically. In this study, hyperosmotic stress was considered as an elicitor. METHODS: Using two fluorescent membrane dyes PKH2 and PKH26, and an automated fluorescence ... More
Proteoliposomes colocalized with endogenous mitochondria in mouse fertilized egg.
AuthorsInoki Y, Hakamata Y, Hamamoto T, Kinouchi T, Yamazaki S, Kagawa Y, Endo H
JournalBiochem Biophys Res Commun
PubMed ID11071871
'Colocalization of mitochondria is the first step of intermitochondrial interaction or fusion in a cell. Here, we showed colocalization between exogenous mitochondria and endogenous ones or between exogenous proteoliposomes and endogenous mitochondria in mouse fertilized eggs by confocal laser microscopy. Isolated mitochondria from mouse liver and proteoliposomes containing mitochondrial membrane ... More
In vitro T lymphopoiesis of human and rhesus CD34+ progenitor cells.
AuthorsRosenzweig M, Marks DF, Zhu H, Hempel D, Mansfield KG, Sehgal PK, Kalams S, Scadden DT, Johnson RP
JournalBlood
PubMed ID8639759
'Differentiation of hematopoietic progenitor cells into T lymphocytes generally occurs in the unique environment of the thymus, a feature that has hindered efforts to model this process in the laboratory. We now report that thymic stromal cultures from rhesus macaques can support T-cell differentiation of human or rhesus CD34+ progenitor ... More
Intrinsic potential of phenotypically defined human hemopoietic stem cells to self-renew in short-term in vitro cultures.
AuthorsKnaän-Shanzer S, Verlinden SF, van Beusechem VW, Van Bekkum DW, Valerio D
JournalExp Hematol
PubMed ID10480435
'In search for culture conditions that will facilitate hemopoietic stem cell (HSC) replication while preserving their primitive properties, we have made use of a multi-parameter FACS assay to define HSCs on basis of their phenotypic characteristics, i.e., CD34++CD33,38,71(-). Bone marrow and umbilical cord blood samples of CD34(+) cells from 31 ... More
Orderly process of sequential cytokine stimulation is required for activation and maximal proliferation of primitive human bone marrow CD34+ hematopoietic progenitor cells residing in G0.
AuthorsLadd AC, Pyatt R, Gothot A, Rice S, McMahel J, Traycoff CM, Srour EF
JournalBlood
PubMed ID9226166
'Bone marrow (BM) CD34+ cells residing in the G0 phase of cell cycle may be the most suited candidates for the examination of cell cycle activation and proliferation of primitive hematopoietic progenitor cells (HPCs). We designed a double simultaneous labeling technique using both DNA and RNA staining with Hoechst 33342 ... More
Intercellular adhesion can be visualized using fluorescently labeled fibrosarcoma HT1080 cells cocultured with hematopoietic cell lines or CD34(+) enriched human mobilized peripheral blood cells.
AuthorsOh DJ, Martinez AR, Lee GM, Francis K, Palsson BO
JournalCytometry
PubMed ID10805931
'BACKGROUND: Intercellular contacts between adjacent cells migrating over each other are important in many cellular processes. However, it has been difficult to visualize and identify dynamic intercellular adhesions between migrating cells in situ. METHODS: Two fluorescent membrane dyes, PKH2 and PKH26 for staining HT1080 and hematopoietic cells and cell lines, ... More
Embryonic neurons transplanted to regions of targeted photolytic cell death in adult mouse somatosensory cortex re-form specific callosal projections.
AuthorsHernit-Grant CS, Macklis JD
JournalExp Neurol
PubMed ID8635560
'In the neocortex, the effectiveness of potential transplantation therapy for diseases involving neuronal loss may depend upon whether donor neurons can reestablish the precise long-distance projections that form the basis of sensory, motor, and cognitive function. During corticogenesis, the formation of these connections is affected by tropic factors, extracellular matrix, ... More
Telomerase activity in candidate stem cells from fetal liver and adult bone marrow.
AuthorsYui J, Chiu CP, Lansdorp PM
JournalBlood
PubMed ID9558381
'Telomerase is a ribonucleoprotein polymerase that synthesizes telomeric repeats onto the 3'' ends of eukaryotic chromosomes. Activation of telomerase may prevent telomeric shortening and correlates with cell immortality in the germline and certain tumor cells. Candidate hematopoietic stem cells (HSC) from adult bone marrow express low levels of telomerase, which ... More
PKH26 probe in the study of the proliferation of chemoresistant leukemic sublines.
AuthorsBoutonnat J, Muirhead KA, Barbier M, Mousseau M, Ronot X, Seigneurin D
JournalAnticancer Res
PubMed ID9891474
'Proliferative status and multidrug resistance status are key predictors of therapeutic outcome in acute myeloid leukemia. Although classical methods for proliferative assessment such as tritiated thymidine or BrdUrd incorporation, are correlated with treatment outcome, they are time consuming and difficult to standardize. As an alternative, we have evaluated the use ... More
Plasticity of retrovirus-labelled myotubes in the newt limb regeneration blastema.
AuthorsKumar A, Velloso CP, Imokawa Y, Brockes JP
JournalDev Biol
PubMed ID10656757
'Two important indices of myogenic differentiation are the formation of syncytial myotubes and the postmitotic arrest from the cell cycle, both of which occur after fusion of mononucleate cells. We show here that these indices are reversed in the environment of the urodele limb regeneration blastema. In order to introduce ... More
Properties of human connexin 31, which is implicated in hereditary dermatological disease and deafness.
AuthorsAbrams CK, Freidin MM, Verselis VK, Bargiello TA, Kelsell DP, Richard G, Bennett MV, Bukauskas FF
JournalProc Natl Acad Sci U S A
PubMed ID16549784
'The connexins are a family of at least 20 homologous proteins in humans that form aqueous channels connecting the interiors of coupled cells and mediating electrical and chemical communication. Mutations in the gene for human connexin 31 (hCx31) are associated with disorders of the skin and auditory system. Alterations in ... More
PKH26 and 125I-PKH95: characterization and efficacy as labels for in vitro and in vivo endothelial cell localization and tracking.
AuthorsFord JW, Welling TH, Stanley JC, Messina LM
JournalJ Surg Res
PubMed ID8606504
'PKH26, a fluorescent cell label, and PKH95, a 125 I-radioactive cell label, are both potentially valuable endothelial cell labels because they bind irreversibly within cell membranes. These labels would be particularly well suited to tract transplanted endothelial cells in vivo. However, no previous studies documenting lack of transfer of the ... More
Migration of human neural stem cells toward an intracranial glioma.
AuthorsJeon JY, An JH, Kim SU, Park HG, Lee MA,
JournalExp Mol Med
PubMed ID18305401
'Many in vivo and in vitro studies have demonstrated the targeted migration of neural stem cells (NSC) to infiltrating brain tumors, including malignant glioma, highlighting a potential therapeutic approach. However, there is not enough information to apply this approach to clinical therapy. The most important things in stem cell therapy ... More
Cell-cell adhesion in limb-formation, estimated from photographs of cell sorting experiments based on a spatial stochastic model.
AuthorsMochizuki A, Wada N, Ide H, Iwasa Y
JournalDev Dyn
PubMed ID9520108
'We developed a new method to estimate the magnitude of differential cell-cell adhesion of two tissues based on the spatial patterns in cell-sorting experiments, and applied it to experimental data on progress-zone cells of avian limb bud at stages 20-26. The change in cell distribution in the experiment was recorded, ... More
Rapid and selective removal of larval erythrocytes from systemic circulation during metamorphosis of the bullfrog, Rana catesbeiana.
AuthorsHasebe T, Oshima H, Kawamura K, Kikuyama S
JournalDev Growth Differ
PubMed ID10545036
'Mechanisms of hemoglobin transition during bullfrog metamorphosis were investigated by labeling red blood cells from larvae (L-RBC) and from froglets (A-RBC) with a fluorescent dye, PKH26. The life span of the labeled L-RBC in systemic circulation was significantly shorter when they were injected into the animals at the metamorphic climax, ... More
In situ labeling of adherent cells with PKH26.
AuthorsLee GM, Fong S, Francis K, Oh DJ, Palsson BO
JournalIn Vitro Cell Dev Biol Anim
PubMed ID10691033
A confocal microscopical analysis of Peyer's patch membranous (M) cell and lymphocyte interactions in the scid mouse.
AuthorsSavidge TC, Smith MW, James PS
JournalAdv Exp Med Biol
PubMed ID8525917
Cell division tracking and expansion of hematopoietic long-term repopulating cells.
AuthorsOostendorp RA, Audet J, Miller C, Eaves CJ
JournalLeukemia
PubMed ID10214853
The combined use of rigorous assays for quantitating transplantable stem cell numbers and precise cell labeling and tracking procedures have provided definitive evidence that stem cell self-renewal divisions can occur in vitro in the absence of stromal feeder layers. These findings set the stage for defining conditions that may alter ... More
A cell line that can induce thymocyte positive selection.
AuthorsHugo P, Kappler JW, Godfrey DI, Marrack PC
JournalNature
PubMed ID1465132
The thymus positively selects thymocytes that bear T-cell receptors which recognize antigen presented by self major histocompatibility complex (MHC) proteins. Positive selection is usually driven by MHC products on radiation-resistant cortical epithelial cells. It is unknown whether positive selection is mediated by all thymic epithelial cells or by some specialized ... More
Nanotubular highways for intercellular organelle transport.
AuthorsRustom A, Saffrich R, Markovic I, Walther P, Gerdes HH
JournalScience
PubMed ID14963329
Cell-to-cell communication is a crucial prerequisite for the development and maintenance of multicellular organisms. To date, diverse mechanisms of intercellular exchange of information have been documented, including chemical synapses, gap junctions, and plasmodesmata. Here, we describe highly sensitive nanotubular structures formed de novo between cells that create complex networks. These ... More
Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1.
AuthorsGazit R, Gruda R, Elboim M, Arnon TI, Katz G, Achdout H, Hanna J, Qimron U, Landau G, Greenbaum E, Zakay-Rones Z, Porgador A, Mandelboim O
JournalNat Immunol
PubMed ID16565719
The elimination of viruses and tumors by natural killer cells is mediated by specific natural killer cell receptors. To study the in vivo function of a principal activating natural killer cell receptor, NCR1 (NKp46 in humans), we replaced the gene encoding this receptor (Ncr1) with a green fluorescent protein reporter ... More
Plasmodium gallinaceum: fluorescent staining of zygotes and ookinetes to study malaria parasites in mosquito.
AuthorsShahabuddin M, Gayle M, Zieler H, Laughinghouse A
JournalExp Parasitol
PubMed ID9538861
We have developed a fluorescent labeling procedure for staining the mosquito stages of Plasmodium gallinaceum. PKH26, a lipophilic dye, is efficiently and permanently incorporated into the membranes of zygotes and ookinetes. Stained zygotes undergo normal development into ookinetes; the stain does not interfere with ookinete mobility or ability to adhere ... More
Stress renders T cell blasts sensitive to killing by activated syngeneic NK cells.
AuthorsRabinovich BA, Shannon J, Su RC, Miller RG
JournalJ Immunol
PubMed ID10946262
Exposure of primary T cell blasts to stress in the forms of heat, hydrogen peroxide, or high-density growth conditions resulted in a state of enhanced susceptibility to killing by syngeneic IL-2-activated NK cells or lymphokine-activated killer cells, but not to killing by CTL. Cytotoxicity was perforin mediated and was not ... More
Tracking of murine spleen cells in vivo: detection of PKH26-labeled cells in the pancreas of non-obese diabetic (NOD) mice.
AuthorsBeavis AJ, Pennline KJ
JournalJ Immunol Methods
PubMed ID8157988
Flow cytometry was used to track the in vivo migration of PKH26-labeled donor spleen cells from diabetic NOD mice that were injected into non-diabetic recipient NOD mice. Flow cytometric analysis of recipient mouse tissues revealed that the donor cells were present in the peripheral blood, spleen and lymph nodes 24 ... More
Localisation of human peripheral blood leukocytes after transfer to C.B-17 scid/scid mice.
AuthorsLadel CH, Kaufmann SH, Bamberger U
JournalImmunol Lett
PubMed ID8300156
Severe combined immunodeficient (scid) mice of the inbred strain C.B-17 lack functional T and B cells and, because of this, they tolerate xenografts. We reconstituted scid mice with human peripheral blood leukocytes (PBL) by i.p. injection. In order to determine the human PBL in lymphoid organs of these reconstituted scid ... More
A flow cytometric rosetting assay for the analysis of Fc receptors and C3 receptors on HSV-infected cells.
AuthorsVan Vliet KE, De Graaf-Miltenburg LA, Verhoef J, Van Strijp JA
JournalJ Immunol Methods
PubMed ID8423374
A sensitive and reproducible flow cytometric assay was developed for the analysis of Fc gamma and C3b(i) receptors on HSV-infected cells. The method is based on a rosette technique using fluorochrome-labeled erythrocytes sensitized with IgG or C3b(i). A comparison of flow cytometric and microscopic quantitation demonstrated that the binding of ... More
Role of CD47 as a marker of self on red blood cells.
The immune system recognizes invaders as foreign because they express determinants that are absent on host cells or because they lack "markers of self" that are normally present. Here we show that CD47 (integrin-associated protein) functions as a marker of self on murine red blood cells. Red blood cells that ... More
A novel mechanism for protein delivery: granzyme B undergoes electrostatic exchange from serglycin to target cells.
AuthorsRaja SM, Metkar SS, Höning S, Wang B, Russin WA, Pipalia NH, Menaa C, Belting M, Cao X, Dressel R, Froelich CJ
JournalJ Biol Chem
PubMed ID15788411
The molecular interaction of secreted granzyme B-serglycin complexes with target cells remains undefined. Targets exposed to double-labeled granzyme B-serglycin complexes show solely the uptake of granzyme B. An in vitro model demonstrates the exchange of the granzyme from serglycin to immobilized, sulfated glycosaminoglycans. Using a combination of cell binding and ... More
Flow patterns of blood cells in the retinal capillaries. Retinal capillary flow patterns.
AuthorsBen-nun J, Alder V, Thompson D, Constable IJ
JournalInt Ophthalmol
PubMed ID1587699
We present a new technique for retinal blood cell angiography which was used to investigate flow patterns of blood cells in the retinal capillary net of normal cats. Capillary paths were defined to be the path taken by the labelled blood cells from the terminal arteriole to the entry point ... More
Flow cytometric quantitation of attachment and phagocytosis in phenotypically-defined subpopulations of cells using PKH26-labeled Fc gamma R-specific probes.
AuthorsPricop L, Salmon JE, Edberg JC, Beavis AJ
JournalJ Immunol Methods
PubMed ID9236915
Human receptors for IgG (Fc gamma R) are characterized by diverse structure and function. We describe a flow cytometric technique to quantitate receptor-specific Fc gamma R-mediated attachment and phagocytosis in phenotypically-defined subsets of cells using erythrocytes (E) labeled with the lipophilic dye PKH26 and coupled with biotin/avidin to either human ... More
Support for the immortal strand hypothesis: neural stem cells partition DNA asymmetrically in vitro.
AuthorsKarpowicz P, Morshead C, Kam A, Jervis E, Ramunas J, Ramuns J, Cheng V, van der Kooy D
JournalJ Cell Biol
PubMed ID16115957
The immortal strand hypothesis proposes that asymmetrically dividing stem cells (SCs) selectively segregate chromosomes that bear the oldest DNA templates. We investigated cosegregation in neural stem cells (NSCs). After exposure to the thymidine analogue 5-bromo-2-deoxyuridine (BrdU), which labels newly synthesized DNA, a subset of neural precursor cells were shown to ... More
Application of ultrasound to selectively localize nanodroplets for targeted imaging and therapy.
AuthorsDayton PA, Zhao S, Bloch SH, Schumann P, Penrose K, Matsunaga TO, Zutshi R, Doinikov A, Ferrara KW,
JournalMol Imaging
PubMed ID16954031
Lipid-coated perfluorocarbon nanodroplets are submicrometer-diameter liquid-filled droplets with proposed applications in molecularly targeted therapeutics and ultrasound (US) imaging. Ultrasonic molecular imaging is unique in that the optimal application of these agents depends not only on the surface chemistry, but also on the applied US field, which can increase receptor-ligand binding ... More
Comparison of the labeling efficiency of BrdU, DiI and FISH labeling techniques in bone marrow stromal cells.
AuthorsLi N, Yang H, Lu L, Duan C, Zhao C, Zhao H,
JournalBrain Res
PubMed ID18468584
Cells are generally labeled during in vivo implantation studies enabling the cells to be traced. The relationship between the labeling efficiency and cellular proliferation after transplantation is critical for the interpretation of data obtained by detection of the signals on tissue sections. Here, we compare cellular labeling methods of rat ... More
Lipophilic dye staining of Cryptococcus neoformans extracellular vesicles and capsule.
AuthorsNicola AM, Frases S, Casadevall A,
JournalEukaryot Cell
PubMed ID19465562
Cryptococcus neoformans is an encapsulated yeast that causes systemic mycosis in immunosuppressed individuals. Recent studies have determined that this fungus produces vesicles that are released to the extracellular environment both in vivo and in vitro. These vesicles contain assorted cargo that includes several molecules associated with virulence and implicated in ... More
Endothelial cell transplantation into the corpus cavernosum: moving towards cell-based gene therapy.
AuthorsWessells H, Williams SK
JournalJ Urol
PubMed ID10569612
PURPOSE: As a first step toward a cell based gene therapy for erectile dysfunction, we transplanted fluorescently labeled autologous microvessel endothelial cells (MVEC) into the rat corpus cavernosum. MATERIALS AND METHODS: MVEC were isolated from the epididymal fat pad, labeled with the membrane intercalating dye PKH 26, and injected into ... More
Sorting out of cells from different parts and stages of the chick limb bud.
AuthorsIde H, Wada N, Uchiyama K
JournalDev Biol
PubMed ID8125199
To examine differences in the surface properties of progress zone cells of avian wing buds, we mixed cells from the "progress zone" at different stages. To identify the origin of cells, a chick-specific antibody was used in a mixed culture of chick and quail cells, or cells from one of ... More
Germ line chimera produced by transfer of cultured chick primordial germ cells.
AuthorsChang IK, Yoshiki A, Kusakabe M, Tajima A, Chikamune T, Naito M, Ohno T
JournalCell Biol Int
PubMed ID7550064
Intrinsic primordial germ cells (PGCs) from stage 27 (5-day-old) chick embryonic germinal ridges were cultured in vitro for a further 5 days, and shown to proliferate on stroma cells derived from the germinal ridge. To determine whether these cultured PGCs could colonize and contribute to the germ-line, PGCs were isolated ... More
Micromechanical control of cell-cell interactions.
AuthorsHui EE, Bhatia SN
JournalProc Natl Acad Sci U S A
PubMed ID17389399
The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By using a micromachined silicon substrate with moving parts, we demonstrate the dynamic regulation of cell-cell interactions via direct manipulation ... More
Molecular mechanisms of erythrophagocytosis: flow cytometric quantitation of in vitro erythrocyte phagocytosis by macrophages.
A rapid, sensitive, and reproducible flow cytofluorimetric procedure is described for quantitation of erythrophagocytosis based on the use of red blood cells (RBCs) labeled with the fluorescent probe PKH-26. The procedure involves the following steps: i) incubation of PKH-26-labeled erythrocytes with macrophages, ii) removal of un-bound red blood cells, iii) ... More
Homing of long-term and short-term engrafting cells in vivo.
AuthorsLanzkron SM, Collector MI, Sharkis SJ
JournalAnn N Y Acad Sci
PubMed ID10372110
Long-term repopulating hematopoietic stem cells can be separated from cells which provided radioprotection (short-term repopulating cells) on the basis of size. This might be a result of the quiescent nature of long-term repopulating cells. To define the activity of these populations we utilized a dye, PKH26, which incorporates into the ... More
Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen.
AuthorsBouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE
JournalProc Natl Acad Sci U S A
PubMed ID16549792
We have developed a vaccine strategy for generating an attenuated strain of an intracellular bacterial pathogen that, after uptake by professional antigen-presenting cells, does not replicate intracellularly and is readily killed. However, after degradation of the vaccine strain within the phagolysosome, target antigens are released into the cytosol for endogenous ... More
Direct evidence of macrophage differentiation from bone marrow cells in the liver: a possible origin of Kupffer cells.
AuthorsTakezawa R, Watanabe Y, Akaike T
JournalJ Biochem (Tokyo)
PubMed ID8720132
Controversy has surrounded origin and differentiation of tissue macrophages. We directly demonstrate the differentiation of bone marrow cells into macrophages in the liver in vivo using a cell-labeling fluorescence dye, PKH-26. Bone marrow cells labeled with PKH26 were intravenously injected into syngenic mice, and these cells were tracked by flow ... More
In vivo labelling of the spleen with a red-fluorescent cell dye.
AuthorsKhalaf AN, Wolff-Vorbeck G, Bross K, Kerp L, Petersen KG
JournalJ Immunol Methods
PubMed ID8409463
A method for labelling mouse spleen cells in situ is described. Spleen vessels were clamped before intrasplenic injection of a red-fluorescent cell dye (PKH-26). The labelling rate was 11.8% of all cells and 13.9% of B lymphocytes 30 min after injection as determined by FACS. 3 days later, the proportions ... More
A novel approach to the measurement of different in vitro leukaemic cell growth parameters: the use of PKH GL fluorescent probes.
AuthorsAshley DM, Bol SJ, Waugh C, Kannourakis G
JournalLeuk Res
PubMed ID7692186
The application of the fluorescent cell membrane probes PKH2 and PKH 26 GL in the measurement of leukaemic cell growth was examined on four cell lines K562, NALM-6, ACV (a pre-B cell line) and HL-60 using flow cytometry. As the amount of probe per cell reduces at each cell division, ... More
A new flow cytometric method for quantitative assessment of lymphocyte mitogenic potentials.
AuthorsYamamura Y, Rodriguez N, Schwartz A, Eylar E, Bagwell B, Yano N
JournalCell Mol Biol (Noisy-le-grand)
PubMed ID8574140
A new flow cytometric method was developed to quantitatively assess lymphocyte proliferation simultaneously for different subsets. The cells were stained with a fluorescent dye, PKH-26 and were stimulated with mitogens. The fluorescence intensities (FL2) of proliferating cells were measured by flow cytometry; and each subset was identified by the use ... More
Intraocular pressure and blood flow of the optic disk: a fluorescent blood cell angiography study.
AuthorsBen-Nun J, Nemet P
JournalSurv Ophthalmol
PubMed ID7660311
The damage to disk microcirculation in glaucoma and ocular hypertension is correlated with the damage to the neuronal tissue. However, it is not clear whether the primary damage is vascular or neurogenic. We have developed a new method which allows in vivo observation of the retinal capillary blood cell flow ... More
Flow cytometric analysis of natural killer cell function as a clinical assay.
AuthorsHatam L, Schuval S, Bonagura VR
JournalCytometry
PubMed ID8033735
The 51Cr release assay has been the method of choice in analyzing natural killer cell (NK) function. Previous FCM cytotoxicity assays of NK activity have had numerous disadvantages that discouraged clinicians from attempting to evaluate NK function by flow cytometry. We demonstrate the effectiveness of using PKH-26, a stable membrane ... More
Regulation of the differentiation of diploid and some aneuploid rat liver epithelial (stemlike) cells by the hepatic microenvironment.
AuthorsColeman WB, Wennerberg AE, Smith GJ, Grisham JW
JournalAm J Pathol
PubMed ID8494041
Following intrahepatic transplantation in adult syngeneic Fischer 344 rats, diploid cultured rat liver epithelial cells (WB-F344), modified to carry the Escherichia coli beta-galactosidase reporter gene and/or the fluorescent membrane dye PKH26-GL, integrate into hepatic plates and acquire the size and nuclear structure of mature hepatocytes. Additionally, of two aneuploid, neoplastically ... More
Difference between dorsal and ventral iris in lens producing potency in normal lens regeneration is maintained after dissociation and reaggregation of cells from the adult newt, Cynops pyrrhogaster.
AuthorsOkamoto M, Ito M, Owaribe K
JournalDev Growth Differ
PubMed ID9563906
In Wolffian lens regeneration, lentectomized newt eye can produce a new lens from the dorsal marginal iris, but the ventral iris has never shown such capabilities. To investigate the difference of lens regenerating potency between dorsal and ventral iris epithelium at the cellular level, a transplantation system using cell reaggregates ... More
Motility and cytoskeletal organization of migrating cerebellar granule neurons.
AuthorsRivas RJ, Hatten ME
JournalJ Neurosci
PubMed ID7869123
To characterize CNS neuronal precursor migration along astroglial fibers, we examined the motility of the migratory leading process and cytoskeletal-based mechanisms of locomotion of early postnatal mouse cerebellar granule neurons in vitro. To visualize the surface motility of the leading process, granule neurons were labeled with the fluorescent lipophilic dye, ... More
Fluorescent dyes for lymphocyte migration and proliferation studies.
AuthorsParish CR
JournalImmunol Cell Biol
PubMed ID10571670
Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as being the ... More
Flow cytometric study of differentiating cultures of Bacillus subtilis.
AuthorsChung JD, Conner S, Stephanopoulos G
JournalCytometry
PubMed ID7587720
We report on 1) the development of a flow cytometry-based technique for detecting beta-galactosidase in differentiating cultures of Bacillus subtilis and 2) the application of this technique in the study of early developmental gene expression. The problems associated with generating detectable signals (despite the small size of B. subtilis cells) ... More
Viability of fibroblast-seeded ligament analogs after autogenous implantation.
AuthorsBellincampi LD, Closkey RF, Prasad R, Zawadsky JP, Dunn MG
JournalJ Orthop Res
PubMed ID9747781
Fibroblast-seeded collagen scaffolds or ligament analogs are potentially useful for reconstruction of the anterior cruciate ligament of the knee. To provide lasting benefits, the seeded cells must survive implantation within the harsh synovial environment of the knee joint. Our objective was to determine the in vivo fate of autogenous fibroblast-seeded ... More
Migration and homing of lymphocytes to lymphoid and synovial tissues in proteoglycan-induced murine arthritis.
AuthorsMikecz K, Glant TT
JournalArthritis Rheum
PubMed ID7945505
OBJECTIVE. To describe the migration and homing of labeled donor lymphocytes to the lymphoid organs and synovial tissues of host animals, during the development of cartilage proteoglycan (PG)-induced arthritis adoptively transferred to syngeneic BALB/c mice. METHODS. Lymphocytes from either nonarthritic or arthritic donor animals were labeled with either fluorescent or ... More
Short-chain alcohols promote an early stage of membrane hemifusion.
AuthorsChanturiya A, Leikina E, Zimmerberg J, Chernomordik LV
JournalBiophys J
PubMed ID10512823
Hemifusion, the linkage of contacting lipid monolayers of two membranes before the opening of a fusion pore, is hypothesized to proceed through the formation of a stalk intermediate, a local and strongly bent connection between membranes. When the monolayers' propensity to bend does not support the stalk (e.g., as it ... More
The lipid-anchored ectodomain of influenza virus hemagglutinin (GPI-HA) is capable of inducing nonenlarging fusion pores.
AuthorsMarkosyan RM, Cohen FS, Melikyan GB
JournalMol Biol Cell
PubMed ID10749920
GPI-linked hemagglutinin (GPI-HA) of influenza virus was thought to induce hemifusion without pore formation. Cells expressing either HA or GPI-HA were bound to red blood cells, and their fusion was compared by patch-clamp capacitance measurements and fluorescence microscopy. It is now shown that under more optimal fusion conditions than have ... More
Changes in the lipophilicity of the surfaces of Meloidogyne incognita and Haemonchus contortus during exposure to host signals.
AuthorsLópez de Mendoza ME, Modha J, Roberts MC, Curtis R, Kusel JR
JournalParasitology
PubMed ID10726281
The surfaces of plant and animal parasitic nematodes share certain lipids, which seem to be important in the infection process. The surfaces of 2 parasitic nematodes, Meloidogyne incognita and Haemonchus contortus, were activated by different pH buffers to allow the insertion of different fluorescent probes. The lipid analogue PKH26 and ... More
Heparan sulfate-mediated cell aggregation. Syndecans-1 and -4 mediate intercellular adhesion following their transfection into human B lymphoid cells.
AuthorsStanley MJ, Liebersbach BF, Liu W, Anhalt DJ, Sanderson RD
JournalJ Biol Chem
PubMed ID7890615
Because syndecans are present at sites of cell-cell contact in vivo it has been hypothesized that they play a role in mediating cell-cell adhesion. However, there has been no direct evidence to support this notion. To address this question, B lymphoid (ARH-77) cells were transfected with the cDNA for murine ... More
Ex vivo PKH26-labelling of lymphocytes for studies of cell migration in vivo.
A prerequisite for studies of cell migration is that the cells of interest can be appropriately labelled and subsequently easily traced. The use of radioisotopes or fluorescent substances that bind covalently to the cell surface, e.g. fluorescein isothiocyanate (FITC) or rhodamine isothiocyanate (RITC), have limitations such as rapid loss of ... More
Mutant RAS inhibits neutrophil but not macrophage differentiation and allows continued growth of neutrophil precursors.
AuthorsDarley RL, Burnett AK
JournalExp Hematol
PubMed ID10560907
Mutational activation of RAS is the most common molecular abnormality in myeloid leukemias. In order to better understand its role in leukemogenesis, we have devised a model based on the multipotent cell line, FDCP-mix. We show that expression of mutant RAS in FDCP-mix strongly inhibits terminal neutrophil differentiation under the ... More
The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction.
Authorsde-Souza W, de-Carvalho TU, de-Melo ET, Soares CP, Coimbra ES, Rosestolato CT, Ferreira SR, Vieira M
JournalBraz J Med Biol Res
PubMed ID9921284
In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein ... More
Transformed and nontransformed human T lymphocytes migrate to skin in a chimeric human skin/SCID mouse model.
AuthorsRosenblatt-Velin N, Arrighi JF, Dietrich PY, Schnuriger V, Masouyé I, Hauser C
JournalJ Invest Dermatol
PubMed ID9406815
To study human T cell migration to human skin in vivo, we grafted severe combined immunodeficient mice with 500-microm thick human skin. Two weeks after grafting, epidermal and dermal structures in the grafts were of human origin. When we intraperitoneally injected grafted mice with clones of the human HUT-78 T ... More
Fate of allogeneic or syngeneic cells in intravenous or portal vein injection: possible explanation for the mechanism of tolerance induction by portal vein injection.
AuthorsZhang Y, Yasumizu R, Sugiura K, Hashimoto F, Amoh Y, Lian Z, Cherry Nishio N, Ikehara S
JournalEur J Immunol
PubMed ID7913037
In this report we examine the fate of donor cells injected via different routes. When PKH-26-labeled C57BL/6 (B6) spleen cells were intravenously (i.v.) injected into BALB/c mice, the donor cells were rejected within 3 days. In contrast, when the same B6 spleen cells were portal venously (p.v.) injected, they were ... More