用于进行流式细胞分析的含 Mitotracker™ Red & Annexin V Alexa Fluor™ 488 的线粒体膜电位细胞凋亡试剂盒

引用和文献 (4)

Invitrogen™

用于进行流式细胞分析的含 Mitotracker™ Red & Annexin V Alexa Fluor™ 488 的线粒体膜电位细胞凋亡试剂盒

这种流式细胞分析产品提供了一种快速且便利的用于检测细胞凋亡的两个特征-磷脂酰丝氨酸外化和线粒体膜电位变化的检测试剂。使用所提供的结合缓冲液中的 Alexa Fluor™ 488 Annexin V 和 MitoTracker™ Red了解更多信息

货号	数量
V35116	1 kit

货号 V35116

价格（CNY)

5,523.00

Ends: 31-Dec-2025

7,339.00

共减 1,816.00 (25%)

1 kit

添加至购物车

1 kit

价格（CNY)

5,523.00

Ends: 31-Dec-2025

7,339.00

共减 1,816.00 (25%)

1 kit

添加至购物车

这种流式细胞分析产品提供了一种快速且便利的用于检测细胞凋亡的两个特征-磷脂酰丝氨酸外化和线粒体膜电位变化的检测试剂。使用所提供的结合缓冲液中的 Alexa Fluor™ 488 Annexin V 和 MitoTracker™ Red CMXRos 染料对一个细胞群染色后，活细胞显示出的绿色荧光很少而红色荧光较强，而凋亡细胞显示出强烈的绿色荧光且红色荧光有所降低。这些细胞群可以很容易地通过流式细胞仪加以区分，氩离子激光器激发的 488 nm 光谱线可用于激发这两种染料。

查看用于流式细胞分析的所有细胞凋亡试验的选择指南。

仅供科研使用。不可用于诊断程序。

激发/发射Mitotracker™ Red 579⁄599、Alexa Fluor™ 488：499⁄521

流式细胞仪激光线路488

适用于（应用）流式细胞分析

适用于（设备）荧光显微镜、流式细胞仪

反应次数50

产品线Alexa Fluor、MitoTracker

产品类型线粒体膜细胞凋亡试剂盒

数量1 kit

运输条件湿冰

偶联物Alexa Fluor™ 488、MitoTracker™ Red

产品规格管、玻片

Unit Size1 kit

内容与储存

含 1 小瓶 Annexin Alexa Fluor™ 488 偶联物 (250 µL)、3 小瓶 MitoTracker™ Red（50 µg/小瓶）、1 瓶膜联蛋白结合缓冲液（5X 溶液，15 mL）和 1 小瓶 DMSO (100 µL)。

避光储存在冷藏冰箱 (2–8°C) 中。

常见问题解答 (FAQ)

What are the fluorescence excitation/emission maxima for the dyes contained in the Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker Red & Alexa Fluor 488 annexin V, for flow cytometry

Alexa Fluor 488 annexin V: 499/521 nm
MitoTracker Red: 579/599 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (4)

引用和文献

Abstract

Galectin 1 modulates plasma cell homeostasis and regulates the humoral immune response.

Authors:Anginot A, Espeli M, Chasson L, Mancini SJ, Schiff C,

Journal:J Immunol

PubMed ID:23616571

'Galectin-1 (GAL1) is an S-type lectin with multiple functions, including the control of B cell homeostasis. GAL1 expression was reported to be under the control of the plasma cell master regulator BLIMP-1. GAL1 was detected at the protein level in LPS-stimulated B cells and was shown to promote Ig secretion ... More

FGF23 activates injury-primed renal fibroblasts via FGFR4-dependent signalling and enhancement of TGF-ß autoinduction.

Authors:Smith ER, Holt SG, Hewitson TD

Journal:Int J Biochem Cell Biol

PubMed ID:28919046

Bone-derived fibroblast growth factor 23 (FGF23) is an important endocrine regulator of mineral homeostasis with effects transduced by cognate FGF receptor (FGFR)1-a-Klotho complexes. Circulating FGF23 levels rise precipitously in patients with kidney disease and portend worse renal and cardiovascular outcomes. De novo expression of FGF23 has been found in the ... More

High Tidal Volume Induces Mitochondria Damage and Releases Mitochondrial DNA to Aggravate the Ventilator-Induced Lung Injury.

Authors:Lin JY, Jing R, Lin F, Ge WY, Dai HJ, Pan L

Journal:Front Immunol

PubMed ID:30018615

This study aimed to determine whether high tidal volume (HTV) induce mitochondria damage and mitophagy, contributing to the release of mitochondrial DNA (mtDNA). Another aim of the present study was to investigate the role and mechanism of mtDNA in ventilator-induced lung injury (VILI) in rats. ... More

Adaptive phenotypic modulations lead to therapy resistance in chronic myeloid leukemia cells.

Authors:Baykal-Köse S, Acikgoz E, Yavuz AS, Gönül Geyik Ö, Ates H, Sezerman OU, Özsan GH, Yüce Z

Journal:PLoS One

PubMed ID:32106243

Tyrosine kinase inhibitor (TKI) resistance is a major problem in chronic myeloid leukemia (CML). We generated a TKI-resistant K562 sub-population, K562-IR, under selective imatinib-mesylate pressure. K562-IR cells are CD34-/CD38-, BCR-Abl-independent, proliferate slowly, highly adherent and form intact tumor spheroids. Loss of CD45 and other hematopoietic markers reveal these cells have ... More