Novex™ Value™ Tris-Glycine Mini Protein Gel, 4 to 12%, 1.0 mm, 10-well - FAQs

查看更多产品信息 Novex™ Value™ Tris-Glycine Mini Protein Gel, 4 to 12%, 1.0 mm, 10-well - FAQs (XV04120PK20)

7 个常见问题解答

我正在以恒定电压进行Tris-甘氨酸凝胶转印，但电流读数高于预期起始电流。可能因为什么？

电流异常升高的最常见原因是转膜缓冲液。如果转膜缓冲液浓度太高，会导致电导率增加和电流升高。如果不小心用Tris-HCl代替了转膜缓冲液所需的Tris base，也会导致高电流。Tris-HCl可使缓冲液pH降低，引起电导率和电流升高，从而导致过热。我们建议检查转膜缓冲液及其试剂成分，然后重新稀释或重新配制缓冲液。

Can Novex WedgeWell gels be stored at room temperature?

No. Novex WedgeWell gels must be stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Do the Novex WedgeWell gels require any specific buffers?

The Novex WedgeWell gels can use standard Tris-Glycine sample and running buffers. For running under native conditions, we recommend using sample and running buffers that do not contain SDS (such as our Native Tris-Glycine premade buffers).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With Novex WedgeWell gels, what in the chemistry changed to allow for a longer shelf life?

We were able to optimize shelf life through a proprietary gel formulation change. Unfortunately, we are unable to provide specific details on the chemical changes.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the orientation of the printing on the cassette for the Novex WedgeWell gels?

We use what we call ‘mirror printing', the same way we do for Bolt gels. This means that when the gel is loaded in the Mini Gel Tank, the text will face in the readable direction (left to right).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can the new Novex WedgeWell gels be run in the XCell SureLock Mini-Cell?

Yes, the new Novex WedgeWell gels can be run in the XCell SureLock Mini-Cell.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I am transferring a Tris-Glycine gel using constant voltage and the current reading is way over the expected starting current. Can you offer some suggestions?

The most common cause of abnormally high current is the transfer buffer. If the transfer buffer is too concentrated, this leads to increased conductivity and current. High current may also occur if Tris-HCl is accidentally substituted for the Tris base required in the transfer buffer. This will again result in low buffer pH and lead to increased conductivity and current and subsequently, overheating. We recommend checking the transfer buffer and its reagent components and re-diluting or remaking the buffer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.