Zymosan A (S. cerevisiae) BioParticles™，荧光素偶联物
Zymosan A (<i>S. cerevisiae</i>) BioParticles&trade;，荧光素偶联物
引用和文献 (23)
Invitrogen™

Zymosan A (S. cerevisiae) BioParticles™，荧光素偶联物

Molecular Probes™ BioParticles™ 产品线包括一系列荧光标记的热或化学灭活的细菌和酵母，其大小、形状和天然抗原性均各异。这些荧光 BioParticles™ 产品一直被荧光显微镜、定量光谱测定法和流式细胞分析用来研究吞噬作用。我们提供的大肠杆菌了解更多信息
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货号数量
Z284110 mg
货号 Z2841
价格（CNY)
3,960.00
10 mg
添加至购物车
数量:
10 mg
价格（CNY)
3,960.00
10 mg
添加至购物车
Molecular Probes™ BioParticles™ 产品线包括一系列荧光标记的热或化学灭活的细菌和酵母，其大小、形状和天然抗原性均各异。这些荧光 BioParticles™ 产品一直被荧光显微镜、定量光谱测定法和流式细胞分析用来研究吞噬作用。

我们提供的大肠杆菌（K-12 菌株）、金黄色葡萄球菌（Wood 菌株，不含蛋白 A）和酵母聚糖（酿酒酵母）BioParticles™ 偶联物共价标记有多种不同的荧光基团（偶联后已特别注意去除游离染料）。与荧光素标记的 BioParticles™ 偶联物的荧光不同（这种荧光在酸性环境中会被部分淬灭），Alexa Fluor™、BODIPY™ FL、四甲基罗丹明和 Texas Red™ 染料偶联物的荧光在 pH 3 至 10 范围内均匀明亮。

BioParticles 规格：
• 标记（激发/发射波长）：荧光素 (∼494/518 nm)
• 颗粒：酵母聚糖（酿酒酵母）
• 提供调理剂

使用 BioParticles 产品
BioParticles™ 偶联物以冻干粉形式提供。每 mg 固体大约 3 x 108 大肠杆菌或金黄色葡萄球菌颗粒，以及每 mg 固体大约 2 x 107 酵母聚糖颗粒。BioParticles™ 偶联物可在选择用于吞噬作用检测的缓冲液中复溶。结合到细胞表面的 BioParticles™ 偶联物（但未内吞）的荧光可以被溴化乙锭、台盼蓝或其他淬灭基团淬灭。除细胞应用之外，荧光 BioParticles™ 偶联物也可在流式细胞术校准参考品分选细菌及酵母突变株时使用。这些小颗粒也可能是光散射研究的有用参考，因为其大小和形状各不相同。

查找更多 BioParticles™ 产品
我们提供了一系列染料标记和未标记的大肠杆菌（K-12 菌株）、金黄色葡萄球菌（Wood 菌株，不含蛋白 A）和酵母聚糖（酿酒酵母）BioParticles™ 产品。查看 Molecular Probes™ 手册中用于追踪受体结合和吞噬作用的探针—第 16.1 节，了解这些产品及其应用。

关于 pH 敏感性内吞作用检测，请参阅 pHrodo™ BioParticles™ 偶联物。

仅供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型FITC（荧光素）
形式实心
数量10 mg
运输条件室温
种属酿酒酵母
适用于（设备）荧光显微镜
产品线BioParticles
产品类型生物颗粒偶联物
pH3 - 10
Unit Size10 mg
内容与储存
储存在冰箱（-5 至 -30°C）中并避光。

常见问题解答 (FAQ)

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can the BioParticles product line be used for?

Fluorescent Bioparticles have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry. We offer E.Coli, S. aureus, and zymosan BioParticles conjugates covalently labeled with a variety of different fluorophores.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (23)

引用和文献
Abstract
MacMARCKS mutation blocks macrophage phagocytosis of zymosan.
Authors:Zhu Z, Bao Z, Li J
Journal:J Biol Chem
PubMed ID:7629059
'A major protein kinase C substrate, MacMARCKS (F52, MPR), was examined for its role in phagocytosis. In macrophage-phagocytosing zymosan particles, MacMARCKS was concentrated around nascent phagosomes as detected by immunofluorescent microscopy. The effector domain of MacMARCKS contains the phosphorylation sites, a calmodulin binding site, as well as a putative actin ... More
Selective inhibition of IgG-mediated phagocytosis in gelsolin-deficient murine neutrophils.
Authors:Serrander L, Skarman P, Rasmussen B, Witke W, Lew DP, Krause KH, Stendahl O, Nüsse O
Journal:J Immunol
PubMed ID:10946270
'Phagocytosis and the microbicidal functions of neutrophils require dynamic changes of the actin cytoskeleton. We have investigated the role of gelsolin, a calcium-dependent actin severing and capping protein, in peripheral blood neutrophils from gelsolin-null (Gsn-) mice. The phagocytosis of complement opsonized yeast was only minimally affected. In contrast, phagocytosis of ... More
CFTR regulates phagosome acidification in macrophages and alters bactericidal activity.
Authors:Di A, Brown ME, Deriy LV, Li C, Szeto FL, Chen Y, Huang P, Tong J, Naren AP, Bindokas V, Palfrey HC, Nelson DJ
Journal:Nat Cell Biol
PubMed ID:16921366
'Acidification of phagosomes has been proposed to have a key role in the microbicidal function of phagocytes. Here, we show that in alveolar macrophages the cystic fibrosis transmembrane conductance regulator Cl- channel (CFTR) participates in phagosomal pH control and has bacterial killing capacity. Alveolar macrophages from Cftr-/- mice retained the ... More
Disease-causing mutations in the cystic fibrosis transmembrane conductance regulator determine the functional responses of alveolar macrophages.
Authors:Deriy LV, Gomez EA, Zhang G, Beacham DW, Hopson JA, Gallan AJ, Shevchenko PD, Bindokas VP, Nelson DJ,
Journal:J Biol Chem
PubMed ID:19837664
'Alveolar macrophages (AMs) play a major role in host defense against microbial infections in the lung. To perform this function, these cells must ingest and destroy pathogens, generally in phagosomes, as well as secrete a number of products that signal other immune cells to respond. Recently, we demonstrated that murine ... More
Comparisons of CapG and gelsolin-null macrophages: demonstration of a unique role for CapG in receptor-mediated ruffling, phagocytosis, and vesicle rocketing.
Authors:Witke W, Li W, Kwiatkowski DJ, Southwick FS
Journal:J Cell Biol
PubMed ID:11514591
'Capping the barbed ends of actin filaments is a critical step for regulating actin-based motility in nonmuscle cells. The in vivo function of CapG, a calcium-sensitive barbed end capping protein and member of the gelsolin/villin family, has been assessed using a null Capg allele engineered into mice. Both CapG-null mice ... More
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