Taq DNA Polymerase, recombinant
<i>Taq</i> DNA Polymerase, recombinant
<i>Taq</i> DNA Polymerase, recombinant
<i>Taq</i> DNA Polymerase, recombinant
Citations & References (3)
Invitrogen™

Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
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Catalog NumberNo. of Reactions
103420461500 Reactions
10342053100 Reactions
10342020500 Reactions
103421785000 Reactions
Catalog number 10342046
Price (CNY)
9,317.00
Each
Add to cart
No. of Reactions:
1500 Reactions
Request bulk or custom format
Price (CNY)
9,317.00
Each
Add to cart
Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

  • A choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
  • For superior PCR performance, DreamTaq DNA Polymerase is recommended.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatStand-alone enzyme
Hot StartNo
No. of Reactions1500 Reactions
Overhang3'-A
PolymeraseTaq DNA Polymerase
Product TypeDNA Polymerase
Quantity3 x 500 Units
Reaction FormatSeparate Components
Shipping ConditionDry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
Concentration5 U/μL
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceLow
PCR MethodqPCR, Standard PCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• Taq DNA Polymerase (5 U/μL), 3 x 100 μL
• 10X PCR buffer, 7.5 mL
• 50 mM MgCl2, 3 mL

Store at -10°C to -30°C in a non-frost-free freezer.
Guaranteed stable for 6 months when stored properly.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all Taq DNA Polymerase, recombinant FAQs

Citations & References (3)

Citations & References
Abstract
alpha-Methylacyl coenzyme A racemase as a tissue biomarker for prostate cancer.
Authors: Rubin Mark A; Zhou Ming; Dhanasekaran Saravana M; Varambally Sooryanarayana; Barrette Terrence R; Sanda Martin G; Pienta Kenneth J; Ghosh Debashis; Chinnaiyan Arul M;
Journal:JAMA
PubMed ID:11926890
'Edited due to space constraints: CONTEXT: Molecular profiling of prostate cancer has led to the identification of candidate biomarkers and regulatory genes. Discoveries from these genome-scale approaches may have applicability in the analysis of diagnostic prostate specimens. OBJECTIVES: To determine the expression and clinical utility of alpha-methylacyl coenzyme A racemase ... More
Molecular cross-talk between the TRAIL and interferon signaling pathways.
Authors: Kumar-Sinha Chandan; Varambally Sooryanarayana; Sreekumar Arun; Chinnaiyan Arul M;
Journal:J Biol Chem
PubMed ID:11677236
TRAIL/APO-2L induces apoptosis in a variety of transformed cells and has potential as an anti-cancer therapeutic. The physiologic role of TRAIL is presumably more complex than merely activating caspase-mediated cell death. To shed light into TRAIL-mediated signaling, we used DNA microarrays to profile gene expression mediated by TRAIL in breast ... More
Association between CYP2C9 genetic variants and anticoagulation-related outcomes during warfarin therapy.
Authors: Higashi Mitchell K; Veenstra David L; Kondo L Midori; Wittkowsky Ann K; Srinouanprachanh Sengkeo L; Farin Fred M; Rettie Allan E;
Journal:JAMA
PubMed ID:11926893
Edited for space constraints: Warfarin is a commonly used anticoagulant that requires careful clinical management to balance the risks of overanticoagulation and bleeding with those of underanticoagulation and clotting. The principal enzyme involved in warfarin metabolism is CYP2C9, and 2 relatively common variant forms with reduced activity have been identified, ... More