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Gibco™
Defined Keratinocyte Serum-Free Medium (SFM)
Defined Keratinocyte Serum-Free Medium (SFM) consistis of Defined Keratinocyte Serum Free Basal Medium that, with the addition of Defined Keratinocyte SFM Growth Supplement, eliminates the need for bovine pituitary extract, offering a defined media solution for the culture of keratinocytes.
| Catalog Number | Quantity |
|---|---|
| 10744019 | 500 mL |
Catalog number 10744019
Price (CNY)
1,995.00
Each
Quantity:
500 mL
Price (CNY)
1,995.00
Each
Defined Keratinocyte SFM is a serum-free defined medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers.
The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM), allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.
Note: This formulation contains some human and animal origin components.
Note: A Defined Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative. Defined Keratinocyte-SFM consists of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeKeratinocyte Cells, Epithelial Cells
Concentration1 X
Product TypeKit
Quantity500 mL
Shelf Life12 Months
ClassificationSerum-free
Culture TypeMammalian Cell Culture
FormLiquid
Serum LevelSerum-free
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red
Unit SizeEach
Contents & Storage
• 500 mL Defined Keratinocyte-SFM Basal Medium; store in refrigerator (2-8°C) and protect from light
• 1 mL Defined Keratinocyte-SFM Growth Supplement; store in freezer (-5°C to -20°C) and protect from light
Complete Medium: store in refrigerator (2-8°C) and protect from light.
• 1 mL Defined Keratinocyte-SFM Growth Supplement; store in freezer (-5°C to -20°C) and protect from light
Complete Medium: store in refrigerator (2-8°C) and protect from light.